Anti-Histone H3 (butyryl K27) antibody (ab241464)
Key features and details
- Rabbit polyclonal to Histone H3 (butyryl K27)
- Suitable for: ChIP, ICC, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (butyryl K27) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (butyryl K27) -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, ICC, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Histone H3 (butyryl K27).
Database link: P68431 -
Positive control
- WB: HeLa, Jurkat, HEK-293 and HepG2 whole cell lysates, treated with 30 mM sodium butyrate for 4 hours. ICC/IF: HeLa cells (treated with 30mM sodium butyrate for 4 hours). ICC: HeLa cells (treated with 30mM sodium butyrate for 4 hours). ChIP: Chromatin prepared from HeLa cells (treated with 30mM sodium butyrate for 4 hours).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - Anti-Histone H3 (butyryl K27) antibody (ab241464)All lanes : Anti-Histone H3 (butyryl K27) antibody (ab241464) at 1/2000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 5 : HeLa whole cell lysate, untreated
Lane 6 : Jurkat whole cell lysate, untreated
Lane 7 : HEK-293 whole cell lysate, untreated
Lane 8 : HepG2 whole cell lysate, untreated
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 15 kDa
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Immunocytochemistry - Anti-Histone H3 (butyryl K27) antibody (ab241464)HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) labeling Histone H3 (butyryl K27) with ab241464 at 1/30 dilution in ICC.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (butyryl K27) antibody (ab241464)HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) labeling Histone H3 (butyryl K27) with ab241464 at 1/15 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor®488-conjugated Goat anti-Rabbit IgG (H+L).
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ChIP - Anti-Histone H3 (butyryl K27) antibody (ab241464)HeLa (human epithelial cell line from cervix adenocarcinoma; 106, treated with 30mM sodium butyrate for 4 hours) cells were treated with micrococcal nuclease, sonicated, and immunoprecipitated with 5 μg ab241464 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
