Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP90] to N Cadherin - C-terminal
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-N Cadherin antibody [SP90] - C-terminal
    See all N Cadherin primary antibodies

  • Description

    Rabbit monoclonal [SP90] to N Cadherin - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human mesothelioma, liver, adrenal gland fallopian tube, renal cell carcinoma, stomach, Human heart, Mouse liver, and Rat liver tissue; Flow Cyt: HeLa cells.

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling N Cadherin with Purified ab225719 at 1/100 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue sections labeling N Cadherin with Purified ab225719 at 1/100 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human heart tissue sections labeling N Cadherin with Purified ab225719 at 1/100 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling N Cadherin with Purified ab225719 at 1/100 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Flow Cytometry - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)
    Flow Cytometry - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

    Flow cytometry analysis of Hela (human cervix adenocarcinoma) labeling N Cadherin with purified ab225719 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

    Formalin-fixed, paraffin-embedded human mesothelioma tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

  • Flow Cytometry - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)
    Flow Cytometry - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

    Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling N Cadherin with ab225719 (green) compared with an isotype control rabbit IgG (blue). 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

    Formalin-fixed, paraffin-embedded human mesothelioma tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

    Formalin-fixed, paraffin-embedded human liver tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

    Formalin-fixed, paraffin-embedded human adrenal gland tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

    Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

    Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

    Formalin-fixed, paraffin-embedded human stomach tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

  • Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)
    Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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