Anti-TGF beta Receptor II antibody [EPR23237-2] (ab270440)
![Anti-TGF beta Receptor II antibody [EPR23237-2] (ab270440)](https://www.abcam.com/ps/products/270/ab270440/Images/ab270440-1-anti-tgf-beta-receptor-ii-antibody-epr232372-immunocytochemistry-ht1080.jpg "Anti-TGF beta Receptor II antibody [EPR23237-2] (ab270440)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23237-2] to TGF beta Receptor II
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-TGF beta Receptor II antibody [EPR23237-2]
See all TGF beta Receptor II primary antibodies -
Description
Rabbit monoclonal [EPR23237-2] to TGF beta Receptor II -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HT-1080 cell. Flow Cyt: HT-1080 cell.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies.
For details on our patents, please refer to www.abcam.com/RabMAbPatents
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23237-2 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-TGF beta Receptor II antibody [EPR23237-2] (ab270440)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HT-1080 (Human fibrosarcoma cell line) cells labelling TGF beta receptor II with ab270440 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and membranous staining in HT-1080 cell line. Negative control: MCF-7 (Human breast adenocarcinoma cell line). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (Red). Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Flow Cytometry - Anti-TGF beta Receptor II antibody [EPR23237-2] (ab270440)](https://www.abcam.com/ps/products/270/ab270440/Images/ab270440-2-anti-tgf-beta-receptor-ii-antibody-epr232372-flow-cytometry-mcf7.jpg)
Flow Cytometry - Anti-TGF beta Receptor II antibody [EPR23237-2] (ab270440)Flow cytometric analysis of permeabilized MCF7 (Human breast adenocarcinoma epithelial cell - Left) and HT-1080 (Human fibrosarcoma cell - Right) cells labelling TGF beta receptor II with ab270440 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: MCF-7 cells.
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![Anti-TGF beta Receptor II antibody [EPR23237-2] (ab270440)](https://www.abcam.com/ps/products/270/ab270440/Images/ab270440-3-benefits-of-recombinant-antibodies.png)
Anti-TGF beta Receptor II antibody [EPR23237-2] (ab270440)
