Anti-TGF beta Receptor II antibody [EPR23148-21] (ab269279)
![Anti-TGF beta Receptor II antibody [EPR23148-21] (ab269279)](https://www.abcam.com/ps/products/269/ab269279/Images/ab269279-388358-anti-tgf-beta-receptor-iiantibody-epr2314821-western-blot-mouse-lung-mouse-lung-treated-with-pngase-f-lysates.jpg "Anti-TGF beta Receptor II antibody [EPR23148-21] (ab269279)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23148-21] to TGF beta Receptor II
- Suitable for: WB, IP
- Reacts with: Mouse
Overview
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Product name
Anti-TGF beta Receptor II antibody [EPR23148-21]
See all TGF beta Receptor II primary antibodies -
Description
Rabbit monoclonal [EPR23148-21] to TGF beta Receptor II -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseWB Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse lung, Mouse spleen, Hepa1-6, bEnd.3, EL4.IL-2, Mouse thymus and Mouse lung, Mouse lung treated with PNGase F tissue lysates. IP: Mouse lung tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23148-21 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TGF beta Receptor II antibody [EPR23148-21] (ab269279)All lanes : Anti-TGF beta Receptor II antibody [EPR23148-21] (ab269279) at 1/1000 dilution
Lane 1 : Mouse lung tissue lysate
Lane 2 : Mouse lung tissue lysate treated with PNGase F
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 65 kDa
Observed band size: 80/70/67 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile observed is consistent with what has been described in the literature (PMID: 25323858).
Two bands of 80 and 70KDa are glycosylated forms. The band of 67KDa is de-glycosylated form.
Exposure time: 3 minutes
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![Immunoprecipitation - Anti-TGF beta Receptor II antibody [EPR23148-21] (ab269279)](https://www.abcam.com/ps/products/269/ab269279/Images/ab269279-1-anti-tgf-beta-receptor-iiantibody-epr2314821-immunoprecipitation-mouse-lung-tissue-lysate-10-ug.jpg)
Immunoprecipitation - Anti-TGF beta Receptor II antibody [EPR23148-21] (ab269279)TGF beta Receptor II was immunoprecipitated from 0.35 mg Mouse lung tissue lysate with ab269279 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab269279 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.
Lane 1: Mouse lung tissue lysate 10 ug
Lane 2: ab269279 IP in Mouse lung tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab269279 in mouse lung tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
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![Western blot - Anti-TGF beta Receptor II antibody [EPR23148-21] (ab269279)](https://www.abcam.com/ps/products/269/ab269279/Images/ab269279-388357-anti-tgf-beta-receptor-iiantibody-epr2314821-western-blot-mouse-lung-mouse-spleen-hepa16-bend3el4il2-mouse-thymus-lysates.jpg)
Western blot - Anti-TGF beta Receptor II antibody [EPR23148-21] (ab269279)All lanes : Anti-TGF beta Receptor II antibody [EPR23148-21] (ab269279) at 1/1000 dilution
Lane 1 : Mouse lung tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate
Lane 4 : bEnd.3 (mouse brain endothelioma ) whole cell lysate
Lane 5 : EL4.IL-2 (mouse lymphoma t lymphocyte) whole cell lysate
Lane 6 : Mouse thymus tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 65 kDa
Observed band size: 80/70 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile observed is consistent with what has been described in the literature (PMID: 25323858).
Exposure time: Lanes 1-4: 70 seconds;Lanes 5-6: 3 minutes.
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![Anti-TGF beta Receptor II antibody [EPR23148-21] (ab269279)](https://www.abcam.com/ps/products/269/ab269279/Images/ab269279-4-benefits-of-recombinant-antibodies.png)
Anti-TGF beta Receptor II antibody [EPR23148-21] (ab269279)
