Anti-TGF beta Receptor II antibody [EPR14673] (ab184948)
![Anti-TGF beta Receptor II antibody [EPR14673] (ab184948)](https://www.abcam.com/ps/products/184/ab184948/Images/ab184948-367581-anti-tgf-beta-receptor-ii-antibody-epr14673-western-blot-knockout-hepg2-ht-1080-og-a549-wt-empty-human-lysates.jpg "Anti-TGF beta Receptor II antibody [EPR14673] (ab184948)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14673] to TGF beta Receptor II
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-TGF beta Receptor II antibody [EPR14673]
See all TGF beta Receptor II primary antibodies -
Description
Rabbit monoclonal [EPR14673] to TGF beta Receptor II -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2 and A549 and HT-1080 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14673 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TGF beta Receptor II antibody [EPR14673] (ab184948)All lanes : Anti-TGF beta Receptor II antibody [EPR14673] (ab184948) at 1/2000 dilution
Lane 1 : Wild-type A549 whole cell lysate at 40 µg
Lane 2 : TGFBR2 knockout A549 whole cell lysate at 40 µg
Lane 3 : HepG2 whole cell lysate at 20 µg
Lane 4 : HT-1080 whole cell lysate at 20 µg
Predicted band size: 65 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab184948 observed at 80 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab184948 was shown to specifically react with in wild-type A549 cells as signal was lost in TGFBR2 knockout cells. Wild-type and TGFBR2 knockout samples were subjected to SDS-PAGE. Ab184948 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-TGF beta Receptor II antibody [EPR14673] (ab184948)](https://www.abcam.com/ps/products/184/ab184948/Images/ab184948-217646-anti-tgf-beta-receptor-ii-antibody-epr14673-western-blot.jpg)
Western blot - Anti-TGF beta Receptor II antibody [EPR14673] (ab184948)All lanes : Anti-TGF beta Receptor II antibody [EPR14673] (ab184948) at 1/5000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 65 kDa
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![Anti-TGF beta Receptor II antibody [EPR14673] (ab184948)](https://www.abcam.com/ps/products/184/ab184948/Images/ab184948-3-benefits-of-recombinant-antibodies.png)
Anti-TGF beta Receptor II antibody [EPR14673] (ab184948)
