Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PCTAIRE1 antibody [EPR13210] (ab181208)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13210] to PCTAIRE1
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PCTAIRE1 antibody [EPR13210]
    See all PCTAIRE1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13210] to PCTAIRE1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • BxPC-3, Human testis and Human fetal heart cell lysates; BxPC-3 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13210

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)
    Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: PCTAIRE1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HEK293 cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab181208 observed at 63 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab181208 was shown to recognize PCTAIRE1 when PCTAIRE1 knockout samples were used, along with additional cross-reactive bands. Wild-type and PCTAIRE1 knockout samples were subjected to SDS-PAGE. ab181208 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)
    Flow Cytometry - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)

    Flow cytometric analysis of BxPC-3 cells (2% paraformaldehyde-fixed) labeling PCTAIRE1 with ab181208 at 1/20 dilution (red) or a rabbit IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as a secondary antibody.

  • Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)
    Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)
    All lanes : Anti-PCTAIRE1 antibody [EPR13210] (ab181208) at 1/1000 dilution

    Lane 1 : Human testis cell lysate
    Lane 2 : Human fetal heart cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 56 kDa



    These two bands may be the longer form of PCTAIRE1 and the alternatively spliced form.

  • Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)
    Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)
    Anti-PCTAIRE1 antibody [EPR13210] (ab181208) at 1/1000 dilution + BxPC-3 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 56 kDa



    These two bands may be the longer form of PCTAIRE1 and the alternatively spliced form.

  • Anti-PCTAIRE1 antibody [EPR13210] (ab181208)
    Anti-PCTAIRE1 antibody [EPR13210] (ab181208)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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