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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases

Anti-TGF beta Receptor II (phospho S225) antibody [EPR12198] - BSA and Azide free (ab236124)

Anti-TGF beta Receptor II (phospho S225) antibody [EPR12198] - BSA and Azide free (ab236124)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12198] to TGF beta Receptor II (phospho S225) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-TGF beta Receptor II (phospho S225) antibody [EPR12198] - BSA and Azide free
    See all TGF beta Receptor II primary antibodies
  • Description

    Rabbit monoclonal [EPR12198] to TGF beta Receptor II (phospho S225) - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Lysate from HepG2 cells treated with TGF-ß and untreated HepG2 cells.
  • General notes

    Ab236124 is the carrier-free version of ab183037. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab236124 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12198
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • SMADs
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Surface Molecules

Images

  • Western blot - Anti-TGF beta Receptor II (phospho S225) antibody [EPR12198] - BSA and Azide free (ab236124)
    Western blot - Anti-TGF beta Receptor II (phospho S225) antibody [EPR12198] - BSA and Azide free (ab236124)
    All lanes : Anti-TGF beta Receptor II (phospho S225) antibody [EPR12198] (ab183037) at 1/10000 dilution

    Lane 1 : Lysate from HepG2 cells treated with TGF-ß
    Lane 2 : Lysate from HepG2 cells treated with TGF-ß then treated with alkaline phosphatase
    Lane 3 : Lysate from HepG2 cells

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 65 kDa
    Additional bands at: 70-80 kDa (possible glycosylated form)



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183037).

  • Anti-TGF beta Receptor II (phospho S225) antibody [EPR12198] - BSA and Azide free (ab236124)
    Anti-TGF beta Receptor II (phospho S225) antibody [EPR12198] - BSA and Azide free (ab236124)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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