Anti-TGF beta Receptor II antibody (ab186838)
Key features and details
- Rabbit polyclonal to TGF beta Receptor II
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TGF beta Receptor II antibody
See all TGF beta Receptor II primary antibodies -
Description
Rabbit polyclonal to TGF beta Receptor II -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseIHC-P HumanWB MouseRatHuman
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Immunogen
Recombinant fragment within Human TGF beta Receptor II aa 23-280. The exact sequence is proprietary.
Database link: P37173 -
Positive control
- WB: HT-29 and A-549 cell lysates, mouse lung, spleen, brain and kidney tissues, rat lung tissue lysates; IHC: human breast cancer and human appendix; IF: NIH/3T3 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta Receptor II antibody (ab186838)
Paraffin-embedded human breast cancer tissue stained for TGF beta Receptor II using ab186838 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-TGF beta Receptor II antibody (ab186838)Immunofluorescence staining of NIH/3T3 cells stained for TGF beta Receptor II with ab186838 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
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Western blot - Anti-TGF beta Receptor II antibody (ab186838)All lanes : Anti-TGF beta Receptor II antibody (ab186838) at 1/1000 dilution
Lane 1 : HT-29 cell lysate
Lane 2 : A-549 cell lysate
Lane 3 : Mouse lung tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Mouse brain tissue lysate
Lane 6 : Mouse kidney tissue lysate
Lane 7 : Rat lung tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 65 kDa
Exposure time: 3 secondsBlocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta Receptor II antibody (ab186838)Paraffin-embedded human appendix tissue stained for TGF beta Receptor II using ab186838 at 1/100 dilution in immunohistochemical analysis.
