Human BCR knockout HEK-293T cell lysate (ab257858)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260370 - Human BCR knockout HEK293T cell lysate 1 x 100µg ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
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Western blot - Human BCR knockout HEK293T cell lysate (ab257858)
Lane 1: Wild-type HEK-293T cell lysate 20 ug
Lane 2: BCR knockout HEK-293T cell lysate 20 ugLanes 1 - 2: Merged signal (red and green). Green - ab187831 observed at 150 kDa. Red - loading control, ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.
ab187831 was shown to react with BCR in wild-type HEK-293T cells in western blot. The bands observed in BCR knockout cell line ab266583 (BCR knockout cell lysate ab257858) below 150kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and BCR knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab187831 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Human BCR knockout HEK293T cell lysate (ab257858)Lane 1: Wild-type HEK-293T cell lysate 20 ug
Lane 2: BCR knockout HEK-293T cell lysate 20 ugLanes 1 - 2: Merged signal (red and green). Green - ab222406 observed at 150 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab222406 was shown to react with BCR in wild-type HEK-293T cells in western blot. The bands observed in BCR knockout cell line ab266583 (BCR knockout cell lysate ab257858) below 150kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and BCR knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab222406 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human BCR knockout HEK293T cell lysate (ab257858)Allele-1: 1 bp insertion in exon1
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Sanger Sequencing - Human BCR knockout HEK293T cell lysate (ab257858)Allele-2: 2 bp insertion in exon1