Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PKR (phospho T451) antibody [EPR2152Y] - BSA and Azide free (ab227983)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2152Y] to PKR (phospho T451) - BSA and Azide free
  • Suitable for: WB, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-PKR (phospho T451) antibody [EPR2152Y] - BSA and Azide free
    See all PKR primary antibodies

  • Description

    Rabbit monoclonal [EPR2152Y] to PKR (phospho T451) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    ab81303 only detects PKR phosphorylated on Threonine 451.

  • Tested Applications & Species

    Application Species
    Dot
    Pig
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa cells treated with Interferon-a and Calyculin A, whole cell lysate.

  • General notes

    ab227983 is the carrier-free version of ab81303 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab227983 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-PKR (phospho T451) antibody [EPR2152Y] - BSA and Azide free (ab227983)
    Dot Blot - Anti-PKR (phospho T451) antibody [EPR2152Y] - BSA and Azide free (ab227983)

    Dot blot analysis of PKR (pT451) phospho peptide (Lane 1), PKR non-phospho peptide (Lane 2), labeling PKR (phospho T451) with ab81303 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81303).

  • Western blot - Anti-PKR (phospho T451) antibody [EPR2152Y] - BSA and Azide free (ab227983)
    Western blot - Anti-PKR (phospho T451) antibody [EPR2152Y] - BSA and Azide free (ab227983)
    All lanes : Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303) at 1/2000 dilution

    Lane 1 : Untreated HeLa (human cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma) treated with Interferon-a and Calyculin A whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 62 kDa



    Purified format.

    Blocking buffer: 5% NFDM/TBST

    Diluting buffer: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81303).

  • Anti-PKR (phospho T451) antibody [EPR2152Y] - BSA and Azide free (ab227983)
    Anti-PKR (phospho T451) antibody [EPR2152Y] - BSA and Azide free (ab227983)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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