Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Thursday March 04, 2021

Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2152Y] to PKR (phospho T451)
Suitable for: Dot blot, WB
Reacts with: Human, Pig

Product name

Anti-PKR (phospho T451) antibody [EPR2152Y]
See all PKR primary antibodies
Description

Rabbit monoclonal [EPR2152Y] to PKR (phospho T451)
Host species

Rabbit
Specificity

ab81303 only detects PKR phosphorylated on Threonine 451.
Tested Applications & Species

Application Species
Dot
Pig

WB
Human

See all applications and species data
Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Thr451 of Human PKR.
Positive control
- WB: HeLa cell lysates.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
Dot	Pig
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2152Y
Isotype

IgG
Research areas

Western blot - Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303)

All lanes : Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303) at 1/2000 dilution

Lane 1 : Untreated HeLa (human cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma) treated with Interferon-a and Calyculin A whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 62 kDa

Purified format.

Blocking buffer: 5% NFDM/TBST

Diluting buffer: 5% NFDM/TBST
Western blot - Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303)

All lanes : Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303) at 0.048 µg/ml

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa treated with 1000U/ml IFN a1 for 18 hours and then treated with 100nM Calyculin A for 15 minutes whole cell lysate
Lane 3 : HeLa treated with 1000U/ml IFN a1 for 18 hours and then treated with 100nM Calyculin A for 15 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase.

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.01 µg/ml

Predicted band size: 62 kDa

Blocking/Diluting Buffer and concentration: 5% NFDM /TBST
Western blot - Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303)

All lanes : Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303) at 1/2000 dilution (undiluted)

Lane 1 : HeLa cell lysate un-treated
Lane 2 : HeLa cell lysate treated with IFN-alpha

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 62 kDa
Observed band size: 68 kDa
why is the actual band size different from the predicted?
Dot Blot - Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303)

Dot blot analysis of PKR (pT451) phospho peptide (Lane 1), PKR non-phospho peptide (Lane 2), labeling PKR (phospho T451) with ab81303 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes
Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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