Recombinant human IRE1 protein (Active) (ab271544)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, Avi tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human IRE1 protein (Active)
See all IRE1 proteins and peptides -
Biological activity
Assay was done in a kinase buffer using MBP (NEB, 0.1 mg/ml) as a substrate with 20 μM ATP at 30°C for 45 mins. The amount of ATP transferred was calculated using Kinase-Glo reagent.
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Purity
>= 90 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
LSMHQQQQLQHQQFQKELEKIQLLQQQQQQLPFHPPGDTAQDGELLDTSG PYSESSGTSSPSTSPRASNHSLCSGSSASKAGSSPSLEQDDGDEETSVVI VGKISFCPKDVLGHGAEGTIVYRGMFDNRDVAVKRILPECFSFADREVQL LRESDEHPNVIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEP ITLLQQTTSGLAHLHSLNIVHRDLKPHNILISMPNAHGKIKAMISDFGLC KKLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKENPTYTVDIFSAGCVFY YVISEGSHPFGKSLQRQANILLGACSLDCLHPEKHEDVIARELIEKMIAM DPQKRPSAKHVLKHPFFWSLEKQLQFFQDVSDRIEKESLDGPIVKQLERG GRAVVKMDWRENITVPLQTDLRKFRTYKGGSVRDLLRAMRNKKHHYRELP AEVRETLGSLPDDFVCYFTSRFPHLLAHTYRAMELCSHERLFQPYYFHEP PEPQPPVTPDAL -
Predicted molecular weight
62 kDa -
Amino acids
469 to 977 -
Tags
His tag N-Terminus , Avi tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 2.04% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human IRE1 protein (Active) (ab271544)Specific activity of ab271544.
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SDS-PAGE - Recombinant human IRE1 protein (Active) (ab271544)SDS-PAGE analysis of 2 µg ab271544.