Mouse Haptoglobin ELISA Kit (ab272472)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.91 pg/ml
- Range: 18.75 pg/ml - 1200 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Haptoglobin ELISA Kit
See all Haptoglobin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 2.5% Inter-assay Sample n Mean SD CV% Serum 3 2.8% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.91 pg/ml -
Range
18.75 pg/ml - 1200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 115 113% - 117% Urine 116 114% - 118% Serum 110 107% - 113% Hep Plasma 109 104% - 113% EDTA Plasma 106 103% - 108% Cit plasma 111 110% - 111% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Haptoglobin ELISA kit (ab272472) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse Haptoglobin protein in serum, urine, plasma and cell and tissue supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Haptoglobin with 3.91 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Haptoglobin, encoded by the HP gene and produced mostly by hepatic cells, is a major acute phase protein. Haptoglobin is known to bind to free hemoglobin released from erythrocytes with high affinity and inhibits its oxidative activity to allow hepatic recycling of the heme iron. Biosynthesis of haptoglobin can also occur in adipose and lung tissues to provide antioxidant and antimicrobial activity. Studies have shown haptoglobin to play a role in stimulation of angiogenesis and changes in the measured concentrations in serum may associate with certain inflammations, infections, and hemolytic conditions. Human, rat, cow, monkey share 81%, 90%, 76%, and 80% sequence homology with mouse haptoglobin, respectively.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Haptoglobin Capture Antibody 1 x 600µl 10X Mouse Haptoglobin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse Haptoglobin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Anhaptoglobinemia -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Binding peptide
- BP
- Haptoglobin alpha chain
see all -
Database links
- Entrez Gene: 15439 Mouse
- SwissProt: Q61646 Mouse
- Unigene: 26730 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Haptoglobin standard curve in Sample Diluent NS.The Haptoglobin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Haptoglobin in mouse serum and plasma samples.The concentrations of Haptoglobin were measured in duplicates, interpolated from the Haptoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:250,000, plasma (citrate) 1:1,000,000, plasma (EDTA) 1:250,000, and plasma (heparin) 1:250,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Haptoglobin concentration was determined to be 271.1 µg/mL in serum, 836.8 µg/mL in plasma (citrate), 265.2 µg/mL in plasma (EDTA), and 282.0 µg/mL in plasma (heparin).
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Interpolated concentrations of native Haptoglobin in mouse tissue culture supernatant and urine samples.The concentrations of Haptoglobin were measured in duplicates, interpolated from the Haptoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: liver supernatant 1:200 and urine 1:160. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Haptoglobin concentration was determined to be 211.7 ng/mL in liver supernatant and 109.3 ng/mL in urine.
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Sandwich ELISA - Mouse Haptoglobin ELISA Kit (ab272472)To learn more about the advantages of recombinant antibodies see here.
