Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Haptoglobin antibody [EPR22856-212] - BSA and Azide free (ab261918)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22856-212] to Haptoglobin - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Haptoglobin antibody [EPR22856-212] - BSA and Azide free
    See all Haptoglobin primary antibodies

  • Description

    Rabbit monoclonal [EPR22856-212] to Haptoglobin - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody is not recommended for human IHC-P.

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IHC-Frmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse serum, mouse plasma, mouse liver, mouse lung, rat plasma, rat liver, human serum, human plasma and human liver lysates. IHC-P: Mouse lung, mouse liver and rat liver tissues. IHC-Fr: Mouse lung tissue; rat lung tissue. Flow Cyt: Hepa1-6 cells.

  • General notes

    Ab261918 is the carrier-free version of ab256454. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab261918 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-212] - BSA and Azide free (ab261918)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-212] - BSA and Azide free (ab261918)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Haptoglobin with ab256454 at 1/5000 dilution (0.149 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in rat liver. The section was incubated with ab256454 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256454).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-212] - BSA and Azide free (ab261918)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-212] - BSA and Azide free (ab261918)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Haptoglobin with ab256454 at 1/5000 dilution (0.149 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in mouse liver (PMID: 14697247). The section was incubated with ab256454 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256454).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-212] - BSA and Azide free (ab261918)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-212] - BSA and Azide free (ab261918)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Haptoglobin with ab256454 at 1/5000 dilution (0.149 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in mouse lung (PMID: 14697247). The section was incubated with ab256454 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256454).

  • Flow Cytometry - Anti-Haptoglobin antibody [EPR22856-212] - BSA and Azide free (ab261918)
    Flow Cytometry - Anti-Haptoglobin antibody [EPR22856-212] - BSA and Azide free (ab261918)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Hepa1-6 (mouse hepatoma epithelial cell) cells labeling Haptoglobin with ab256454 at 1/700 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256454).

  • Immunohistochemistry (Frozen sections) - Anti-Haptoglobin antibody [EPR22856-212] - BSA and Azide free (ab261918)
    Immunohistochemistry (Frozen sections) - Anti-Haptoglobin antibody [EPR22856-212] - BSA and Azide free (ab261918)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen section of rat lung tissue labeling Haptoglobin with ab256454 at 1/500 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). The nuclear counter stain is DAPI (blue). Positive staining on alveolar epithelial type II cells of rat lung (PMID: 19501987).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256454).

  • Immunohistochemistry (Frozen sections) - Anti-Haptoglobin antibody [EPR22856-212] - BSA and Azide free (ab261918)
    Immunohistochemistry (Frozen sections) - Anti-Haptoglobin antibody [EPR22856-212] - BSA and Azide free (ab261918)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen section of mouse lung tissue labeling Haptoglobin with ab256454 at 1/500 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). The nuclear counter stain is DAPI (blue). Positive staining on alveolar epithelial type II cells and bronchi of mouse lung (PMID: 19501987).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256454).

  • Anti-Haptoglobin antibody [EPR22856-212] - BSA and Azide free (ab261918)
    Anti-Haptoglobin antibody [EPR22856-212] - BSA and Azide free (ab261918)

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