Anti-ARMET/ARP antibody [EPR22538-206] - BSA and Azide free (ab254207)
![Anti-ARMET/ARP antibody [EPR22538-206] - BSA and Azide free (ab254207)](https://www.abcam.com/ps/products/254/ab254207/Images/ab254207-3-253243IP1.jpg "Anti-ARMET/ARP antibody [EPR22538-206] - BSA and Azide free (ab254207)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22538-206] to ARMET/ARP - BSA and Azide free
- Suitable for: WB, ICC/IF, Flow Cyt, IP
- Reacts with: Human
Overview
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Product name
Anti-ARMET/ARP antibody [EPR22538-206] - BSA and Azide free
See all ARMET/ARP primary antibodies -
Description
Rabbit monoclonal [EPR22538-206] to ARMET/ARP - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HeLa cells. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate.
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General notes
ab254207 is the carrier-free version of ab253243 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab254207 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as ARMET
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22538-206 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-ARMET/ARP antibody [EPR22538-206] - BSA and Azide free (ab254207)
ARMET/ARP was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab253243 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab253243 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
Lane 1: HeLa whole cell lysate 10 µg (Input).
Lane 2: ab253243 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253243 in HeLa whole cell lysate.Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 15 seconds.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253243).
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![Flow Cytometry - Anti-ARMET/ARP antibody [EPR22538-206] - BSA and Azide free (ab254207)](https://www.abcam.com/ps/products/254/ab254207/Images/ab254207-2-253243FC1.jpg)
Flow Cytometry - Anti-ARMET/ARP antibody [EPR22538-206] - BSA and Azide free (ab254207)Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling ARMET/ARP with ab253243 at 1/600 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253243). -
![Immunocytochemistry/ Immunofluorescence - Anti-ARMET/ARP antibody [EPR22538-206] - BSA and Azide free (ab254207)](https://www.abcam.com/ps/products/254/ab254207/Images/ab254207-1-253243ICC1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ARMET/ARP antibody [EPR22538-206] - BSA and Azide free (ab254207)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling ARMET/ARP with ab253243 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in HeLa cell line. The nuclear counterstain is DAPI (blue). Counterstained with ab12223 Anti-KDEL antibody - Endoplasmic reticulum Marker (Mouse monoclonal) ab150120 AlexaFluor®594 Goat anti-Mouse secondary.
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253243).
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![Anti-ARMET/ARP antibody [EPR22538-206] - BSA and Azide free (ab254207)](https://www.abcam.com/ps/products/254/ab254207/Images/ab254207-4-benefits-of-recombinant-antibodies.png)
Anti-ARMET/ARP antibody [EPR22538-206] - BSA and Azide free (ab254207)
