Anti-Heme Oxygenase 1 antibody (ab79854)
Key features and details
- Rabbit polyclonal to Heme Oxygenase 1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Heme Oxygenase 1 antibody
See all Heme Oxygenase 1 primary antibodies -
Description
Rabbit polyclonal to Heme Oxygenase 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Multi-antigenic synthetic peptide from Human Heme Oxygenase 1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody (ab79854)
Immunocytochemistry/Immunofluorescence analysis using ab79854 at 1/100 for 12 hours at 4°C in Heat Shocked HeLa Cells. Fixed by 2% Formaldehyde for 20 min at RT. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1/200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at RT. (A) DAPI (blue) nuclear stain. (B) Anti-HO-1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
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Western blot - Anti-Heme Oxygenase 1 antibody (ab79854)All lanes : Anti-Heme Oxygenase 1 antibody (ab79854) at 1 µg/ml
Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from human A431, A549, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC3 and T98G cells
Predicted band size: 33 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody (ab79854)Immunocytochemistry/Immunofluorescence analysis of heat shocked HeLa cells using ab79854 at 1/100 dilution for 12 hours at 4°C. Fixation: 2% Formaldehyde for 20 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytoplasm. Magnification: 100x. Heat Shocked at 42°C for 1h.
