All lanes : Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926) at 1/1000 dilution

Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : MAPK12 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Human skeletal muscle tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

Lanes 1-3: Merged signal (red and green). Green - ab205926 observed at 42 kDa. Red - loading control ab8245 observed at 36 kDa.

 ab205926 Anti-MAPK 12 antibody [EPR6528(N)] was shown to specifically react with MAPK 12 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266280 (knockout cell lysate ab258041) was used. Wild-type and MAPK 12 knockout samples were subjected to SDS-PAGE. ab205926 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

Allele-1: 5 bp deletion in exon 4

 

Allele-2: 1 bp insertion in exon 4.