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Anti-Haptoglobin antibody [EPR22856-233] - BSA and Azide free (ab261725)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22856-233] to Haptoglobin - BSA and Azide free
  • Suitable for: IHC-Fr, WB, IHC-P
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Haptoglobin antibody [EPR22856-233] - BSA and Azide free
    See all Haptoglobin primary antibodies

  • Description

    Rabbit monoclonal [EPR22856-233] to Haptoglobin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse serum, plasma, liver and lung lysates. IHC-P: Mouse lung, liver and cerebral cortex tissues.

  • General notes

    Ab261725 is the carrier-free version of ab256455. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab261725 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-Haptoglobin antibody [EPR22856-233] - BSA and Azide free (ab261725)
    Immunohistochemistry (Frozen sections) - Anti-Haptoglobin antibody [EPR22856-233] - BSA and Azide free (ab261725)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling Haptoglobin with ab256455 at 1/500 (1.17 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256455).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-233] - BSA and Azide free (ab261725)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-233] - BSA and Azide free (ab261725)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Haptoglobin with ab256455 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on bronchi of mouse lung (PMID: 14697247). The section was incubated with ab256455 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256455).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-233] - BSA and Azide free (ab261725)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-233] - BSA and Azide free (ab261725)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Haptoglobin with ab256455 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in mouse liver (PMID: 14697247). The section was incubated with ab256455 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256455).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-233] - BSA and Azide free (ab261725)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-233] - BSA and Azide free (ab261725)

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling Haptoglobin with ab256455 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: no staining on mouse cerebral cortex (PMID: 14697247). The section was incubated with ab256455 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256455).

  • Anti-Haptoglobin antibody [EPR22856-233] - BSA and Azide free (ab261725)
    Anti-Haptoglobin antibody [EPR22856-233] - BSA and Azide free (ab261725)

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