Anti-Glycophorin A antibody [EPR8199] (ab134111)
![Anti-Glycophorin A antibody [EPR8199] (ab134111)](https://www.abcam.com/ps/products/134/ab134111/Images/ab134111-148301-anti-glycophorin-a-antibody-epr8199-western-blot.jpg "Anti-Glycophorin A antibody [EPR8199] (ab134111)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8199] to Glycophorin A
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Glycophorin A antibody [EPR8199]
See all Glycophorin A primary antibodies -
Description
Rabbit monoclonal [EPR8199] to Glycophorin A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region within Human Glycophorin A.
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Positive control
- Human fetal liver lysate, Human lung tissue, Human spleen tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.90 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8199 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Glycophorin A antibody [EPR8199] (ab134111)Anti-Glycophorin A antibody [EPR8199] (ab134111) at 1/1000 dilution + Human fetal liver lysate at 10 µg
Secondary
Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 16 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8199] (ab134111)](https://www.abcam.com/ps/products/134/ab134111/Images/ab134111-148300-anti-glycophorin-a-antibody-epr8199-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8199] (ab134111)Immunohistochemical analysis of paraffin embedded Human lung tissue labelling Glycophorin A with ab134111 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8199] (ab134111)](https://www.abcam.com/ps/products/134/ab134111/Images/ab134111-148299-anti-glycophorin-a-antibody-epr8199-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8199] (ab134111)Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling Glycophorin A with ab134111 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-Glycophorin A antibody [EPR8199] (ab134111)](https://www.abcam.com/ps/products/134/ab134111/Images/ab134111-148298-anti-glycophorin-a-antibody-epr8199.jpg)
OI-RD Scanning - Anti-Glycophorin A antibody [EPR8199] (ab134111) -
![Anti-Glycophorin A antibody [EPR8199] (ab134111)](https://www.abcam.com/ps/products/134/ab134111/Images/ab134111-5-benefits-of-recombinant-antibodies.png)
Anti-Glycophorin A antibody [EPR8199] (ab134111)
