Anti-PKR antibody (ab226819)
Key features and details
- Rabbit polyclonal to PKR
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PKR antibody
See all PKR primary antibodies -
Description
Rabbit polyclonal to PKR -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee
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Immunogen
Recombinant fragment within Human PKR (internal sequence). The exact sequence is proprietary.
Database link: P19525 -
Positive control
- WB: MCF 7 and MDA-MB-231 cell lysate. IP: MCF7 cell lysate. IHC: Human lung cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PKR antibody (ab226819)All lanes : Anti-PKR antibody (ab226819) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line) cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 62 kDa
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Immunoprecipitation - Anti-PKR antibody (ab226819)PKR was immunoprecipitated from MCF7 (human breast adenocarcinoma cell line) whole cell lysate with ab226819. Western blot was performed from the immunoprecipitate using ab226819.
Lane 1: Rabbit IgG instead of ab226819 in MCF7 whole cell lysate.
Lane 2: ab226819 IP in MCF7 whole cell lysate. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKR antibody (ab226819)Paraffin embedded human lung cancer tissue stained for PKR using ab226819 at 1/500 dilution in immunohistochemical analysis.
