Anti-PKR (phospho T446) antibody [E120] (ab32036)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E120] to PKR (phospho T446)
- Suitable for: IHC-P, WB, IP
- Reacts with: Human, Pig
Overview
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Product name
Anti-PKR (phospho T446) antibody [E120]
See all PKR primary antibodies -
Description
Rabbit monoclonal [E120] to PKR (phospho T446) -
Host species
Rabbit -
Specificity
The antibody only detects PKR phosphorylated on Threonine 446. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P PigWB Human -
Immunogen
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Positive control
- WB: HeLa treated with Calyculin A and TNF-alpha whole cell lysate; Calynculin A and IFN treated HeLa cell lysate. IHC-P: Human colon tissue.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E120 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PKR (phospho T446) antibody [E120] (ab32036) at 1/2000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma) treated with 100 ng/mL Calyculin A whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma) treated with 100 ng/mL Calyculin A followed by 20 ng/mL TNF alpha whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking/Diluting buffer: 5% NFDM/TBST
Positive control: Rabbit monoclonal [Y117] to PKR (ab32506).
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Immunohistochemical analysis of PKR (phospho T446) expression in normal colon tissue, using 1/50 unpurified ab32036. Heat mediated antigen retrieval performed before IHC staining.
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All lanes : Anti-PKR (phospho T446) antibody [E120] (ab32036) at 1/1000 dilution (unpurified)
Lane 1 : Hela (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Hela cell lysate + IFN + CalyculinA
Lane 3 : Hela cell lysate + Pptase
Predicted band size: 62 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
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