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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases

Anti-PKR (phospho T446) antibody [E120] (ab32036)

Price and availability

371 894 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-PKR (phospho T446) antibody [E120] (ab32036)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E120] to PKR (phospho T446)
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human, Pig

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Overview

  • Product name

    Anti-PKR (phospho T446) antibody [E120]
    See all PKR primary antibodies
  • Description

    Rabbit monoclonal [E120] to PKR (phospho T446)
  • Host species

    Rabbit
  • Specificity

    The antibody only detects PKR phosphorylated on Threonine 446.
  • Tested Applications & Species

    Application Species
    IHC-P
    Pig
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human PKR. The exact sequence is proprietary.
    Database link: P19525
    (Peptide available as ab181660)

  • Positive control

    • WB: HeLa treated with Calyculin A and TNF-alpha whole cell lysate; Calynculin A and IFN treated HeLa cell lysate. IHC-P: Human colon tissue.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    E120
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases

Images

  • Western blot - Anti-PKR (phospho T446) antibody [E120] (ab32036)
    Western blot - Anti-PKR (phospho T446) antibody [E120] (ab32036)
    All lanes : Anti-PKR (phospho T446) antibody [E120] (ab32036) at 1/2000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma) treated with 100 ng/mL Calyculin A whole cell lysate
    Lane 3 : HeLa (human cervix adenocarcinoma) treated with 100 ng/mL Calyculin A followed by 20 ng/mL TNF alpha whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 62 kDa
    Observed band size: 68 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking/Diluting buffer: 5% NFDM/TBST

    Positive control: Rabbit monoclonal [Y117] to PKR (ab32506).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKR (phospho T446) antibody [E120] (ab32036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKR (phospho T446) antibody [E120] (ab32036)

    Immunohistochemical analysis of PKR (phospho T446) expression in normal colon tissue, using 1/50 unpurified ab32036. Heat mediated antigen retrieval performed before IHC staining.

  • Western blot - Anti-PKR (phospho T446) antibody [E120] (ab32036)
    Western blot - Anti-PKR (phospho T446) antibody [E120] (ab32036)
    All lanes : Anti-PKR (phospho T446) antibody [E120] (ab32036) at 1/1000 dilution (unpurified)

    Lane 1 : Hela (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : Hela cell lysate + IFN + CalyculinA
    Lane 3 : Hela cell lysate + Pptase

    Predicted band size: 62 kDa
    Observed band size: 68 kDa why is the actual band size different from the predicted?

  • Anti-PKR (phospho T446) antibody [E120] (ab32036)
    Anti-PKR (phospho T446) antibody [E120] (ab32036)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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