PE Anti-PKR antibody [YE350] (ab224922)
![PE Anti-PKR antibody [YE350] (ab224922)](https://www.abcam.com/ps/products/224/ab224922/Images/ab224922-312653-anti-pkr-antibody-ye350-phycoerythrin-flow-cytometry.jpg "PE Anti-PKR antibody [YE350] (ab224922)")
Key features and details
- PE Rabbit monoclonal [YE350] to PKR
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG
Overview
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Product name
PE Anti-PKR antibody [YE350]
See all PKR primary antibodies -
Description
PE Rabbit monoclonal [YE350] to PKR -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: HeLa cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
YE350 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - PE Anti-PKR antibody [YE350] (ab224922)
Overlay histogram showing HeLa cells stained with ab224922 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab224922, 1/1000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
