Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TGF beta Receptor II antibody [EPR14673] - BSA and Azide free (ab236136)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14673] to TGF beta Receptor II - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-TGF beta Receptor II antibody [EPR14673] - BSA and Azide free
    See all TGF beta Receptor II primary antibodies

  • Description

    Rabbit monoclonal [EPR14673] to TGF beta Receptor II - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2, HT-1080 and A549 cell lysates.

  • General notes

    Ab236136 is the carrier-free version of ab184948. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab236136 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TGF beta Receptor II antibody [EPR14673] - BSA and Azide free (ab236136)
    Western blot - Anti-TGF beta Receptor II antibody [EPR14673] - BSA and Azide free (ab236136)
    All lanes : Anti-TGF beta Receptor II antibody [EPR14673] (ab184948) at 1/2000 dilution

    Lane 1 : Wild-type A549 whole cell lysate at 40 µg
    Lane 2 : TGFBR2 knockout A549 whole cell lysate at 40 µg
    Lane 3 : HepG2 whole cell lysate at 20 µg
    Lane 4 : HT-1080 whole cell lysate at 20 µg

    Predicted band size: 65 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab184948 observed at 80 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab184948 was shown to specifically react with  in wild-type A549 cells as signal was lost in TGFBR2 knockout cells. Wild-type and TGFBR2 knockout samples were subjected to SDS-PAGE. Ab184948 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184948).

  • Western blot - Anti-TGF beta Receptor II antibody [EPR14673] - BSA and Azide free (ab236136)
    Western blot - Anti-TGF beta Receptor II antibody [EPR14673] - BSA and Azide free (ab236136)
    All lanes : Anti-TGF beta Receptor II antibody [EPR14673] (ab184948) at 1/5000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 65 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184948).

  • Anti-TGF beta Receptor II antibody [EPR14673] - BSA and Azide free (ab236136)
    Anti-TGF beta Receptor II antibody [EPR14673] - BSA and Azide free (ab236136)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-TGF beta Receptor II antibody [EPR14673] - BSA and Azide free (ab236136)

© 2021 Astana Biomed Group LLP. All rights reserved.