Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] - BSA and Azide free (ab187407)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4567(2)] to GDF11 + GDF8/Myostatin - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] - BSA and Azide free
    See all GDF11 + GDF8/Myostatin primary antibodies

  • Description

    Rabbit monoclonal [EPR4567(2)] to GDF11 + GDF8/Myostatin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab187407 is the carrier-free version of ab124721 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab187407 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as BMP11, BMP11 + GDF8 / Myostatin

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Dissociation constant (KD)

    KD = 6.40 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR4567(2)

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] - BSA and Azide free (ab187407)
    Flow Cytometry - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] - BSA and Azide free (ab187407)

    ab124721 staining GDF11 + GDF8/Myostatin in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/250. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124721).

  • Immunocytochemistry/ Immunofluorescence - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] - BSA and Azide free (ab187407)
    Immunocytochemistry/ Immunofluorescence - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] - BSA and Azide free (ab187407)

    Immunocytochemistry/Immunofluorescence analysis of A549 cells labelling BMP11 + GDF8/Myostatin with purified ab124721 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. ab150078, an Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/250) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124721).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] - BSA and Azide free (ab187407)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] - BSA and Azide free (ab187407)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling BMP11 + GDF8/Myostatin with purified ab124721 at 1/2500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124721).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] - BSA and Azide free (ab187407)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] - BSA and Azide free (ab187407)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon adenocarcinoma tissue labelling BMP11 + GDF8/Myostatin with unpurified ab124721 at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124721).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] - BSA and Azide free (ab187407)
    OI-RD Scanning - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] - BSA and Azide free (ab187407)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124721).

  • Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] - BSA and Azide free (ab187407)
    Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] - BSA and Azide free (ab187407)

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