Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22709-52] to Iroquois homeobox protein 3/IRX3 - BSA and Azide free
  • Suitable for: IHC-P, WB, Flow Cyt, ICC
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free
    See all Iroquois homeobox protein 3/IRX3 primary antibodies

  • Description

    Rabbit monoclonal [EPR22709-52] to Iroquois homeobox protein 3/IRX3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, Flow Cyt, ICCmore details
    Unsuitable for: IHC-Fr or IP

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse embryo, E14.5 lung and E17 lung tissue lysates; Rat E17 brain (primary culture) tissue lysate. IHC-P: Mouse E14.5 heart and E14.5 lung tissue; Rat E14.5 heart tissue. ICC: HEK-293T cells. Flow cyt: HEK-293T cells (transfected with Flag-tagged IRX3 expression vector).

  • General notes

    ab274328 is the carrier-free version of ab242133. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22709-52

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
    Immunocytochemistry - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)

    This data was developed using ab242133, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T cells (transfected with Flag-tagged IRX3 expression vector) labelling Iroquois homeobox protein 3/IRX3 with ab242133 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in 293T cells transfected with Flag-tagged IRX3 expression vector. is observed. Anti-FLAG mouse monoclonal antibody was used at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)

    This data was developed using ab242133, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse E14.5 lung tissue labeling Iroquois homeobox protein 3/IRX3 with ab242133 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on lung of mouse E14.5 (PMID: 11520674 ). The section was incubated with ab242133 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Flow Cytometry - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
    Flow Cytometry - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)

    This data was developed using ab242133, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) (transfected with Flag-tagged IRX3 expression vector) cells labelling Iroquois homeobox protein 3/IRX3 with ab242133 at 1/500 dilution (0.1ug)(Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
    Western blot - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
    All lanes : Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133) at 1/1000 dilution

    Lane 1 : Mouse E17 embryo tissue lysate
    Lane 2 : Rat E17 brain (primary culture) tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 52 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab242133, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 125 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)

    This data was developed using ab242133, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat E14.5 heart tissue labeling Iroquois homeobox protein 3/IRX3 with ab242133 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on heart of rat E14.5. The section was incubated with ab242133 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
    Western blot - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
    All lanes : Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133) at 1/1000 dilution

    Lane 1 : Mouse embryo tissue lysate
    Lane 2 : Mouse E14.5 lung tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 52 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    This data was developed using ab242133, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)

    This data was developed using ab242133, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse E14.5 heart tissue labeling Iroquois homeobox protein 3/IRX3 with ab242133 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on heart of mouse E14.5 (PMID: 15342473, PMID: 11520674). The section was incubated with ab242133 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
    Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)

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