Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Rabbit polyclonal to Lin28
Suitable for: Flow Cyt, IHC-P, WB, ICC/IF, IP
Reacts with: Human
Isotype: IgG

Product name

Anti-Lin28 antibody
See all Lin28 primary antibodies
Description

Rabbit polyclonal to Lin28
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human Lin28 aa 1-209. Expressed in bacteria.
Database link: Q9H9Z2
Positive control
- NTERA-2, NCCIT and HeLa whole cell lysates; H9 embryonic stem cells; HEL 11.4 induced IPS cells; Human tonsil and Human placenta tissues; Human embryonal carcinoma NTERA-2 cells; HeLa cells.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 30% Glycerol (glycerin, glycerine), 69% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Western blot - Anti-Lin28 antibody (ab175352)

All lanes : Anti-Lin28 antibody (ab175352) at 1/1000 dilution

Lane 1 : NCCIT whole cell lysate
Lane 2 : NTERA-2 whole cell lysate
Lane 3 : HeLa whole cell lysate

Lysates/proteins at 75 µg per lane.

Secondary
All lanes : goat anti-rabbit-HRP secondary antibody at 1/20000 dilution

Predicted band size: 22 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Lin28 antibody (ab175352)

Immunofluorescent analysis of formalin-fixed and permeabilized HeLa cells labeling Lin28 with ab175352 at 1/100 and incubated with a DyLight-conjugated secondary antibody. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.
Immunocytochemistry/ Immunofluorescence - Anti-Lin28 antibody (ab175352)

Immunofluorescent analysis of fH9 embryonic stem cells labeling Lin28 with ab175352 at 1/200 and incubated with a fluorescently-conjugated secondary antibody. Nuclei (blue) were stained with DAPI.
Immunocytochemistry/ Immunofluorescence - Anti-Lin28 antibody (ab175352)

Immunofluorescent analysis of HEL 11.4 induced IPS cells labeling Lin28 with ab175352 at 1/200 and incubated with a fluorescently-conjugated secondary antibody. Nuclei (blue) were stained with DAPI.
Immunocytochemistry/ Immunofluorescence - Anti-Lin28 antibody (ab175352)

Immunofluorescent analysis of formalin-fixed and permeabilized HeLa cells (left image) and Human embryonal carcinoma NTERA-2 cells (right image) labeling Lin28 with ab175352 at 1/200 and and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody. Nuclei (blue) were stained with Hoechst 33342 dye.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28 antibody (ab175352)

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Lin28 with ab175352 at 1/50.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28 antibody (ab175352)

Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling Lin28 with ab175352 at 1/50.
Flow Cytometry - Anti-Lin28 antibody (ab175352)

Flow Cytometrical analysis of H9 embryonic stem cells labeling Lin28 with ab175352 at 1/100 (right histogram) compared to rabbit IgGl (left histogram) and incubated with a fluorescently-conjugated secondary antibody.
Flow Cytometry - Anti-Lin28 antibody (ab175352)

Flow Cytometrical analysis of HEL 11.4 induced IPS cells labeling Lin28 with ab175352 at 1/100 (right histogram) compared to rabbit IgGl (left histogram) and incubated with a fluorescently-conjugated secondary antibody.
Immunoprecipitation - Anti-Lin28 antibody (ab175352)

Lane 2: Immunoprecipitation. ab175352 at 1/1000 labeling Lin28 immunoprecipitated from 750 μg of NCCIT whole cell lysate using ab175352 at 3μg.

Anti-Lin28 antibody (ab175352) at 1/1000 dilution + NCCIT whole cell lysate

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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