Anti-Lin28 antibody [EPR4639] (ab109751)
![Anti-Lin28 antibody [EPR4639] (ab109751)](https://www.abcam.com/ps/products/109/ab109751/Images/ab109751-122747-anti-lin28-antibody-epr4639-western-blot.jpg "Anti-Lin28 antibody [EPR4639] (ab109751)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4639] to Lin28
- Suitable for: ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-Lin28 antibody [EPR4639]
See all Lin28 primary antibodies -
Description
Rabbit monoclonal [EPR4639] to Lin28 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: JAR and NCCIT cell lysates; ICC: NCCIT cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4639 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Lin28 antibody [EPR4639] (ab109751)All lanes : Anti-Lin28 antibody [EPR4639] (ab109751) at 1/1000 dilution
Lane 1 : JAR cell lysate
Lane 2 : NCCIT cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 23 kDa
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![Immunocytochemistry - Anti-Lin28 antibody [EPR4639] (ab109751)](https://www.abcam.com/ps/products/109/ab109751/Images/ab109751-2-anti-lin28-antibody-epr4639-immunocytochemistry-nccit1.jpg)
Immunocytochemistry - Anti-Lin28 antibody [EPR4639] (ab109751)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NCCIT cells labelling Lin28 with ab109751 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing cytoplasmic staining in NCCIT cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: PBS instead of the primary antibody.
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![Anti-Lin28 antibody [EPR4639] (ab109751)](https://www.abcam.com/ps/products/109/ab109751/Images/ab109751-3-benefits-of-recombinant-antibodies.png)
Anti-Lin28 antibody [EPR4639] (ab109751)
