Mouse Chordin ELISA Kit is designed for the quantitative determination of Chordin in cell culture supernatants, plasma and serum samples.

ΔNote: Use Citrate and EDTA as anti-coagulant. Heparin is not recommended.

This assay employs an antibody specific for mouse Chordin coated on a 96-well plate. Standards and samples are pipetted into the wells and Chordin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Chordin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Chordin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse 4-1BB, 6Ckine, ACE, ALK-1, AREG, CT-1 , CD27, CD27 Ligand, CD36, CD40 Ligand, CTLA 4, Decorin, DKK-1, E Cadherin, EGF, Endoglin, Epigen, EPR, Galectin 1, GAS 1, GAS 6, GITR Ligand, Granzyme B, HAI-1, HGF, IL-1R4, IL-11, IL-17B, IL-17E, IL-17 F, IL-1RA, IL-2R alpha, IL-20, IL-21, IL-28, IL-6R, JAM-A, MFG-E8, NEP, PTX3, PRL, RAGE, TACI, TREM, TWEAK.

• Neuroscience
• Neurology process
• Neurogenesis

• Stem Cells
• Lineage Markers
• Ectoderm

• Developmental Biology
• Embryogenesis
• Morphogens

• Developmental Biology
• Lineage specification
• Ectoderm