Mouse Chordin ELISA Kit (ab267579)
Key features and details
- Sensitivity: 49 pg/ml
- Range: 49.15 pg/ml - 12000 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Chordin ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Cell culture media, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
49 pg/ml -
Range
49.15 pg/ml - 12000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 74 68% - 86% Plasma 74 68% - 85% Cell culture media 100 80% - 117% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Chordin ELISA Kit is designed for the quantitative determination of Chordin in cell culture supernatants, plasma and serum samples.
ΔNote: Use Citrate and EDTA as anti-coagulant. Heparin is not recommended.
This assay employs an antibody specific for mouse Chordin coated on a 96-well plate. Standards and samples are pipetted into the wells and Chordin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Chordin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Chordin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse 4-1BB, 6Ckine, ACE, ALK-1, AREG, CT-1 , CD27, CD27 Ligand, CD36, CD40 Ligand, CTLA 4, Decorin, DKK-1, E Cadherin, EGF, Endoglin, Epigen, EPR, Galectin 1, GAS 1, GAS 6, GITR Ligand, Granzyme B, HAI-1, HGF, IL-1R4, IL-11, IL-17B, IL-17E, IL-17 F, IL-1RA, IL-2R alpha, IL-20, IL-21, IL-28, IL-6R, JAM-A, MFG-E8, NEP, PTX3, PRL, RAGE, TACI, TREM, TWEAK.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Mouse Chordin coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Mouse Chordin Detection Antibody 2 vials Mouse Chordin Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes. -
Tissue specificity
Expressed at the highest level in liver. -
Sequence similarities
Belongs to the chordin family.
Contains 4 CHRD domains.
Contains 4 VWFC domains. -
Post-translational
modificationsCleaved by tolloid proteases; cleavage participates to dorsoventral patterning during early development. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Chordin
- chrd
- CHRD_HUMAN
see all -
Database links
- Entrez Gene: 12667 Mouse
- SwissProt: Q9Z0E2 Mouse
- Unigene: 20457 Mouse
Images
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Standard curve
Mouse Chordin ELISA kit (ab267579) Standard curve.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.