Anti-SCA10 antibody (ab231644)
Key features and details
- Rabbit polyclonal to SCA10
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-SCA10 antibody
See all SCA10 primary antibodies -
Description
Rabbit polyclonal to SCA10 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Cynomolgus monkey, Orangutan
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Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human SCA10 aa 100-350. Expressed in E.coli.
Database link: Q9UBB4 -
Positive control
- WB: Recombinant human SCA10 protein; HEK-293T and HeLa cell lysates; pig kidney and brain lysates. IHC-P: Human prostate, prostate cancer, liver, liver cancer and kidney tissue. ICC/IF: HeLa cells.
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General notes
This product was previously labelled as ATXN10
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SCA10 antibody (ab231644)Anti-SCA10 antibody (ab231644) at 1 µg + Recombinant human SCA10 protein
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 53 kDa
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Western blot - Anti-SCA10 antibody (ab231644)All lanes : Anti-SCA10 antibody (ab231644) at 1 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Pig kidney tissue lysate
Lane 4 : Pig brain tissue lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 53 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-SCA10 antibody (ab231644)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SCA10 (green) using ab231644 at 20 μg/mL in ICC/IF. FITC secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCA10 antibody (ab231644)Formalin-fixed, paraffin-embedded human prostate tissue stained for SCA10 using ab231644 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCA10 antibody (ab231644)Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for SCA10 using ab231644 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCA10 antibody (ab231644)Formalin-fixed, paraffin-embedded human liver tissue stained for SCA10 using ab231644 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCA10 antibody (ab231644)Formalin-fixed, paraffin-embedded human liver cancer tissue stained for SCA10 using ab231644 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCA10 antibody (ab231644)Formalin-fixed, paraffin-embedded human kidney tissue stained for SCA10 using ab231644 at 20 μg/ml in immunohistochemical analysis. DAB staining.
