Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hes1 antibody [EPR4226] - BSA and Azide free (ab221788)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4226] to Hes1 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, ICC
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Hes1 antibody [EPR4226] - BSA and Azide free
    See all Hes1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4226] to Hes1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Fetal brain, PC12, and SH-SY5Y cell lysates. Human placenta tissue.

  • General notes

    Ab221788 is the carrier-free version of ab108937. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab221788 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody [EPR4226] - BSA and Azide free (ab221788)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody [EPR4226] - BSA and Azide free (ab221788)

    Primary ab concentration dilution: 1:200, (0.5ug/ml), Secondary ab: ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG, Secondary ab concentration: Prediluted, Tissue: Human brain, Fixative: Paraffin-embedded sections, Counter stain: Hematoxylin Antigen retrieval: Perform heat mediated antigen retrieval using Tris/EDTA Buffer, PH9

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108937).

  • Immunocytochemistry - Anti-Hes1 antibody [EPR4226] - BSA and Azide free (ab221788)
    Immunocytochemistry - Anti-Hes1 antibody [EPR4226] - BSA and Azide free (ab221788)

    Clone EPR4226 (ab221788) has been successfully conjugated by Abcam. This image was generated using Anti-Hes1 antibody [EPR4226] (Alexa Fluor® 488). Please refer to ab196328 for protocol details.

    ab196328 staining Hes1 in SKNSH cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196328 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2 µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Flow Cytometry - Anti-Hes1 antibody [EPR4226] - BSA and Azide free (ab221788)
    Flow Cytometry - Anti-Hes1 antibody [EPR4226] - BSA and Azide free (ab221788)

    Clone EPR4226 (ab221788) has been successfully conjugated by Abcam. This image was generated using Anti-Hes1 antibody [EPR4226] (PE). Please refer to ab225087 for protocol details.

    Overlay histogram showing SHSY5Y cells stained with ab225087 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225087, 1/1000 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody [EPR4226] - BSA and Azide free (ab221788)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody [EPR4226] - BSA and Azide free (ab221788)

    Immunohistochemical staining of Hes1 in paraffin-embedded human placenta tissue with unpurified ab108937 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108937).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody [EPR4226] - BSA and Azide free (ab221788)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody [EPR4226] - BSA and Azide free (ab221788)

    Immunohistochemical staining of paraffin embedded human brain with unpurified ab108937 at a working dilution of 1 in 90. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108937).

  • Immunocytochemistry - Anti-Hes1 antibody [EPR4226] - BSA and Azide free (ab221788)
    Immunocytochemistry - Anti-Hes1 antibody [EPR4226] - BSA and Azide free (ab221788)

    Clone EPR4226 (ab221788) has been successfully conjugated by Abcam. This image was generated using Anti-Hes1 antibody [EPR4226] (Alexa Fluor® 647). Please refer to ab196577 for protocol details.

    ab196577 staining Hes1 in SK-N-SH cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196577 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
    This product also gave a positive signal under the same testing conditions in SK-N-SH cells fixed with 100% methanol (5min).

  • Immunocytochemistry - Anti-Hes1 antibody [EPR4226] - BSA and Azide free (ab221788)
    Immunocytochemistry - Anti-Hes1 antibody [EPR4226] - BSA and Azide free (ab221788)

    Immunofluorescent staining of SH-SY5Y cells (fixed with 4% PFA and permeablized with TritonX 100) with purified ab108937 at a dilution of 1/100. An Alexa Fluor® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108937).

  • Immunocytochemistry - Anti-Hes1 antibody [EPR4226] - BSA and Azide free (ab221788)
    Immunocytochemistry - Anti-Hes1 antibody [EPR4226] - BSA and Azide free (ab221788)

    Immunofluorescent staining of SH-SY5Y cells (fixed with 4% PFA and permeablized with TritonX 100) with unpurified ab108937 at a dilution of 1/40. An Alexa Fluor® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108937).

  • Flow Cytometry - Anti-Hes1 antibody [EPR4226] - BSA and Azide free (ab221788)
    Flow Cytometry - Anti-Hes1 antibody [EPR4226] - BSA and Azide free (ab221788)

    Overlay histogram showing SH-SY5Y cells stained with unpurified ab108937 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab108937, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108937).

  • Anti-Hes1 antibody [EPR4226] - BSA and Azide free (ab221788)
    Anti-Hes1 antibody [EPR4226] - BSA and Azide free (ab221788)

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