Anti-DGAT1 antibody (ab100982)
Key features and details
- Rabbit polyclonal to DGAT1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-DGAT1 antibody
See all DGAT1 primary antibodies -
Description
Rabbit polyclonal to DGAT1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide corresponding to Human DGAT1 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab111783) -
Positive control
- This antibody gave a positive signal in Caco2 whole cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab100982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatCowPigApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).Target
-
Function
Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. -
Pathway
Lipid metabolism; glycerolipid metabolism. -
Sequence similarities
Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
-
Database links
- Entrez Gene: 282609 Cow
- Entrez Gene: 8694 Human
- Entrez Gene: 13350 Mouse
- Entrez Gene: 397118 Pig
- Entrez Gene: 84497 Rat
- Omim: 604900 Human
- SwissProt: Q8MK44 Cow
- SwissProt: O75907 Human
see all -
Alternative names
- ACAT related gene product 1 antibody
- ACAT-related gene product 1 antibody
- Acyl coenzyme A:cholesterol acyltransferase related gene 1 antibody
see all
Images
-
Western blot - Anti-DGAT1 antibody (ab100982)Anti-DGAT1 antibody (ab100982) at 1 µg/ml + Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Additional bands at: 49 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
Datasheets and documents
References (1)
ab100982 has been referenced in 1 publication.
- Guo Z et al. mTORC2 Regulates Lipogenic Gene Expression through PPAR? to Control Lipid Synthesis in Bovine Mammary Epithelial Cells. Biomed Res Int 2019:5196028 (2019). PubMed: 31223619
Images
-
Western blot - Anti-DGAT1 antibody (ab100982)Anti-DGAT1 antibody (ab100982) at 1 µg/ml + Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Additional bands at: 49 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
