Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free (ab240250)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13430-4] to DGAT1 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IP, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free
    See all DGAT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13430-4] to DGAT1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa cell lysate, HEK-293 and Jurkat whole cell lysate prepared in 1%SDS Hot lysis method Human small intestine lysates IP: Human fetal kidney lysate. ICC/IF: MCF7, HeLa cells. Flow Cyt: HeLa cells.

  • General notes

    Ab240250 is the carrier-free version of ab181180. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240250 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free (ab240250)
    Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free (ab240250)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling DGAT1 with Purified ab181180 at 1:100 dilution (4.3 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181180)

  • Flow Cytometry - Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free (ab240250)
    Flow Cytometry - Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free (ab240250)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling DGAT1 with Purified ab181180 at 1:400 dilution (1 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181180)

  • Immunoprecipitation - Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free (ab240250)
    Immunoprecipitation - Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free (ab240250)

    ab181180 (purified) at 1:30 dilution (2µg) immunoprecipitating DGAT1 in Human fetal kidney lysate.
    Lane 1 (input): Human fetal kidney lysate 10µg
    Lane 2 (+): ab181180 & Human fetal kidney lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181180 in Human fetal kidney lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181180)

  • Immunoprecipitation - Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free (ab240250)
    Immunoprecipitation - Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free (ab240250)

    Western blot analysis on immunoprecipitation pellet from Human fetal kidney lysate (lane 1) or negative control (lane 2), labeling DGAT1 immunoprecipitated using ab181180 at 1/1500 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181180).

  • Flow Cytometry - Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free (ab240250)
    Flow Cytometry - Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free (ab240250)

    Flow cytometry analysis of DGAT1 expression in HeLa cells using ab181180 at 1/70 dilution (red) and a rabbit IgG as negative control (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181180).

  • Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free (ab240250)
    Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free (ab240250)

    Immunofluorescence analysis of acetone-fixed HeLa cells, staining DGAT1 (green) with ab181180 at 1/100 dilution. Alexa Fluor®488-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181180).

  • Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free (ab240250)
    Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free (ab240250)

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