Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13430-4] to DGAT1 - N-terminal
  • Suitable for: ICC/IF, IP, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-DGAT1 antibody [EPR13430-4] - N-terminal
    See all DGAT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13430-4] to DGAT1 - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa cell lysate, HEK-293 and Jurkat whole cell lysate prepared in 1%SDS Hot lysis method Human small intestine lysates IP: Human fetal kidney lysate. ICC/IF: MCF7, HeLa cells. Flow Cyt: HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
    Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling DGAT1 with Purified ab181180 at 1:100 dilution (4.3 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
    Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
    All lanes : Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180) at 1/10000 dilution

    Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate prepared using RIPA lysis method
    Lane 2 : HEK-293 whole cell lysate prepared using 1% SDS Hot lysis method
    Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate prepared using RIPA lysis method
    Lane 4 : Jurkat whole cell lysate prepared using 1% SDS Hot lysis method

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa


    Exposure time: 3 seconds


    Blocking buffer and concentration: 5% NFDM/TBST

    We recommend to use 1% SDS Hot lysis method to get clear Western Blot results.

  • Flow Cytometry - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
    Flow Cytometry - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling DGAT1 with Purified ab181180 at 1:400 dilution (1 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunoprecipitation - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
    Immunoprecipitation - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

    ab181180 (purified) at 1:30 dilution (2µg) immunoprecipitating DGAT1 in Human fetal kidney lysate.
    Lane 1 (input): Human fetal kidney lysate 10µg
    Lane 2 (+): ab181180 & Human fetal kidney lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181180 in Human fetal kidney lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
    Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
    Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180) at 1/10000 dilution + Human small intestine tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa


    Exposure time: 3 minutes


    Blocking buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
    Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
    Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180) at 1/50000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
    Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

    Immunofluorescence analysis of acetone-fixed HeLa cells, staining DGAT1 (green) with ab181180 at 1/100 dilution. Alexa Fluor®488-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

  • Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
    Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
    Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180) at 1/10000 dilution + Human fetal kidney lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa

  • Flow Cytometry - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
    Flow Cytometry - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

    Flow cytometry analysis of DGAT1 expression in HeLa cells using ab181180 at 1/70 dilution (red) and a rabbit IgG as negative control (green).

  • Immunoprecipitation - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
    Immunoprecipitation - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

    Western blot analysis on immunoprecipitation pellet from Human fetal kidney lysate (lane 1) or negative control (lane 2), labeling DGAT1 immunoprecipitated using ab181180 at 1/1500 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
    Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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