Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13430-4] to DGAT1 - N-terminal
Suitable for: ICC/IF, IP, Flow Cyt, WB
Reacts with: Human

Product name

Anti-DGAT1 antibody [EPR13430-4] - N-terminal
See all DGAT1 primary antibodies
Description

Rabbit monoclonal [EPR13430-4] to DGAT1 - N-terminal
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa cell lysate, HEK-293 and Jurkat whole cell lysate prepared in 1%SDS Hot lysis method Human small intestine lysates IP: Human fetal kidney lysate. ICC/IF: MCF7, HeLa cells. Flow Cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13430-4
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling DGAT1 with Purified ab181180 at 1:100 dilution (4.3 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

All lanes : Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180) at 1/10000 dilution

Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate prepared using RIPA lysis method
Lane 2 : HEK-293 whole cell lysate prepared using 1% SDS Hot lysis method
Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate prepared using RIPA lysis method
Lane 4 : Jurkat whole cell lysate prepared using 1% SDS Hot lysis method

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution

Predicted band size: 55 kDa
Observed band size: 55 kDa

Exposure time: 3 seconds

Blocking buffer and concentration: 5% NFDM/TBST

We recommend to use 1% SDS Hot lysis method to get clear Western Blot results.
Flow Cytometry - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling DGAT1 with Purified ab181180 at 1:400 dilution (1 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunoprecipitation - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

ab181180 (purified) at 1:30 dilution (2µg) immunoprecipitating DGAT1 in Human fetal kidney lysate.
Lane 1 (input): Human fetal kidney lysate 10µg
Lane 2 (+): ab181180 & Human fetal kidney lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181180 in Human fetal kidney lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180) at 1/10000 dilution + Human small intestine tissue lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution

Predicted band size: 55 kDa
Observed band size: 55 kDa

Exposure time: 3 minutes

Blocking buffer and concentration: 5% NFDM/TBST
Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180) at 1/50000 dilution + HeLa cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 55 kDa
Observed band size: 55 kDa
Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

Immunofluorescence analysis of acetone-fixed HeLa cells, staining DGAT1 (green) with ab181180 at 1/100 dilution. Alexa Fluor®488-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180) at 1/10000 dilution + Human fetal kidney lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

Predicted band size: 55 kDa
Observed band size: 55 kDa
Flow Cytometry - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

Flow cytometry analysis of DGAT1 expression in HeLa cells using ab181180 at 1/70 dilution (red) and a rabbit IgG as negative control (green).
Immunoprecipitation - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

Western blot analysis on immunoprecipitation pellet from Human fetal kidney lysate (lane 1) or negative control (lane 2), labeling DGAT1 immunoprecipitated using ab181180 at 1/1500 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

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