Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)
![Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)](https://www.abcam.com/ps/products/108/ab108429/Images/ab108429-257929-anti-glutathione-peroxidase-1-antibody-epr3311-western-blot.jpg "Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3311] to Glutathione Peroxidase 1
- Suitable for: WB, IHC-P, Flow Cyt
- Knockout validated
- Reacts with: Human
Overview
-
Product name
Anti-Glutathione Peroxidase 1 antibody [EPR3311]
See all Glutathione Peroxidase 1 primary antibodies -
Description
Rabbit monoclonal [EPR3311] to Glutathione Peroxidase 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Human fetal liver, SH SY5Y, and THP1 cell lysates; Human breast carcinoma tissue.
-
General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3311 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: GPX1 knockout HAP1 cell lysate (20 µg)
Lane 3: THP1 cell lysate (20 µg)
Lane 4: HL60 cell lysate (20 µg)
Lanes 1 and 2: Merged signal (red and green). Green - ab108429, observed at 22 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab108429 was shown to specifically react with Glutathione Peroxidase 1 in wild-type HAP1 cells. No band was observed when Glutathione Peroxidase 1 knockout samples were examined. Wild-type and Glutathione Peroxidase 1 knockout samples were subjected to SDS-PAGE. ab108429 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
![Flow Cytometry - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)](https://www.abcam.com/ps/products/108/ab108429/Images/ab108429-278239-anti-glutathione-peroxidase-1-antibody-epr3311-flow-cytometry.jpg)
Flow Cytometry - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)Flow Cytometry analysis of THP-1 (human acute monocytic leukemia ) cells labeling Glutathione Peroxidase 1 with purified ab108429 at 1:250 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
-
![Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)](https://www.abcam.com/ps/products/108/ab108429/Images/ab108429-121162-anti-glutathione-peroxidase-1-antibody-epr3311-western-blot.gif)
Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)All lanes : Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : SH SY5Y cell lysate
Lane 3 : THP1 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 22 kDa
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)](https://www.abcam.com/ps/products/108/ab108429/Images/ab108429-121161-anti-glutathione-peroxidase-1-antibody-epr3311-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)ab108429, at 1/100 dilution, staining Glutathione Peroxidase 1 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)](https://www.abcam.com/ps/products/108/ab108429/Images/ab108429-5-benefits-of-recombinant-antibodies.png)
Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)
