Anti-Glutathione Peroxidase 1 antibody - C-terminal (ab230882)
Key features and details
- Rabbit polyclonal to Glutathione Peroxidase 1 - C-terminal
- Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Glutathione Peroxidase 1 antibody - C-terminal
See all Glutathione Peroxidase 1 primary antibodies -
Description
Rabbit polyclonal to Glutathione Peroxidase 1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Glutathione Peroxidase 1 aa 164-193 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P07203 -
Positive control
- IHC-P: Human liver tissue. ICC/IF: HepG2 cells. WB: THP-1 cell lysate; Mouse and rat liver lysates. Flow Cyt: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Glutathione Peroxidase 1 antibody - C-terminal (ab230882)All lanes : Anti-Glutathione Peroxidase 1 antibody - C-terminal (ab230882) at 1/1000 dilution
Lane 1 : THP-1 (Human monocytic leukemia cell line) cell lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Rat liver tissue lysate
Lysates/proteins at 35 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG H&L (HRP) at 1/5000 dilution
Predicted band size: 22 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Glutathione Peroxidase 1 antibody - C-terminal (ab230882)HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for Glutathione Peroxidase 1 using ab230882 at 1/10 dilution in ICC/IF, followed by Alexa Fluor®488-conjugated Goat Anti-Rabbit IgG (green). DAPI was used to stain the cell nuclear (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 1 antibody - C-terminal (ab230882)Formalin-fixed, paraffin-embedded human liver tissue stained for Glutathione Peroxidase 1 using ab230882 at 1/100 dilution in immunohistochemical analysis.
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Flow Cytometry - Anti-Glutathione Peroxidase 1 antibody - C-terminal (ab230882)Flow cytometric analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, labeling Glutathione Peroxidase 1 with ab230882 at 1/10 dilution (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
