Alexa Fluor® 488 Anti-Cyclophilin F antibody [E11AE12BD4] (ab156032)
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [E11AE12BD4] to Cyclophilin F
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Mouse, Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG1
Overview
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Product name
Alexa Fluor® 488 Anti-Cyclophilin F antibody [E11AE12BD4]
See all Cyclophilin F primary antibodies -
Description
Alexa Fluor® 488 Mouse monoclonal [E11AE12BD4] to Cyclophilin F -
Host species
Mouse -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant full length protein corresponding to Rat Cyclophilin F.
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Positive control
- HDFn and HeLa cells.
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General notes
Product was previously marketed under the MitoSciences sub-brand.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading...
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Purification notes
ab156032 is a conjugated form of ab110324 which was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
E11AE12BD4 -
Isotype
IgG1 -
Research areas
Images
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Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Cyclophilin F with ab156032 at 5.0 µg/ml, (green); Anti-HSP60 (1/2500, ab46798) as red, and DAPI in blue, as a nuclear stain.
Secondary antibody used was goat anti-rabbit Alexa594 (1/1000).
All blocking and antibodies were diluted in 10% normal goat serum. -
Immunofluorescent analysis of paraformaldehyde-fixed HDFn cells labeling Cyclophilin F with ab156032 at 5.0 µg/ml (green); Anti-HSP60 (1/2500, ab46798) as red, and DAPI in blue, as a nuclear stain.
Secondary antibody used was goat anti-rabbit Alexa594 (1/1000).
All blocking and antibodies were diluted in 10% normal goat serum -
Flow cytometric analysis of HeLa cells labeling Cyclophilin F with ab156032 at 0.2 µg/ml (red) or an equal amount of IgG1 Alexa-488 Isotype control antibody (blue).