Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)
![Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)](https://www.abcam.com/ps/products/251/ab251279/Images/ab251279-446507-anti-rab5c-rabl-antibody-epr17321-bsa-and-azide-free-western-blot.jpg "Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17321] to RAB5C/RABL - BSA and Azide free
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free
See all RAB5C/RABL primary antibodies -
Description
Rabbit monoclonal [EPR17321] to RAB5C/RABL - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251279 is the carrier-free version of ab199530 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab251279 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as RAB5C
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR17321 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)All lanes : Anti-RAB5C/RABL antibody [EPR17321] (ab199530) at 1/1000 dilution
Lane 1 : HEK293 (Human embryonic kidney) cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 10 secondsThis data was developed using ab199530, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry - Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)](https://www.abcam.com/ps/products/251/ab251279/Images/ab251279-5-anti-rab5c-rabl-antibody-epr17321-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)This data was developed using ab199530, the same antibody clone in a different buffer formulation.
Ab199530 staining RAB5C/RABL in HepG2 cells (Human hepatocellular carcinoma epithelial cell) by Immunocytochemistry/Immunofluorescence (ICC/IF). Cells were fixed with 100% Methanol. Samples were incubated with primary antibody at 1:500 dilution (2.2µg/ml). An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1:1000 dilution (2µg/ml). An Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594), ab195889 was used as a counterstain antibody at 1:200 dilution (2.5µg/ml). DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic staining in HepG2 cells. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)](https://www.abcam.com/ps/products/251/ab251279/Images/ab251279-3-anti-rab5c-rabl-antibody-epr17321-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)This data was developed using ab199530, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling RAB5C/RABL with ab199530 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining of Human kidney tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody and secondary antibody Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Western blot - Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)](https://www.abcam.com/ps/products/251/ab251279/Images/ab251279-408161-anti-rab5c-rabl-antibody-epr17321-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)Anti-RAB5C/RABL antibody [EPR17321] (ab199530) at 1/10000 dilution + U937 (Human histiocytic lymphoma cells) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 3 minutesThis data was developed using ab199530, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)](https://www.abcam.com/ps/products/251/ab251279/Images/ab251279-405833-anti-rab5c-rabl-antibody-epr17321-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)Anti-RAB5C/RABL antibody [EPR17321] (ab199530) at 1/1000 dilution + Human fetal heart tissue lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 3 minutesThis data was developed using ab199530, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)](https://www.abcam.com/ps/products/251/ab251279/Images/ab251279-4-benefits-of-recombinant-antibodies.png)
Anti-RAB5C/RABL antibody [EPR17321] - BSA and Azide free (ab251279)