Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [7G8D3] (ab233722)

Key features and details

Mouse monoclonal [7G8D3] to Metabotropic Glutamate Receptor 7/MGLUR7
Suitable for: WB, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [7G8D3]
See all Metabotropic Glutamate Receptor 7/MGLUR7 primary antibodies
Description

Mouse monoclonal [7G8D3] to Metabotropic Glutamate Receptor 7/MGLUR7
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human Metabotropic Glutamate Receptor 7/MGLUR7 aa 450-600. (Expressed in E.coli).
Database link: Q14831

Run BLAST with Run BLAST with
Positive control
- WB: Recombinant human Metabotropic Glutamate Receptor 7/MGLUR7 (aa 454-590) protein; Metabotropic Glutamate Receptor 7/MGLUR7 (aa 454-590)-hIgGFc transfected HEK-293 cell lysate. ICC/IF: HeLa and SMMC-7721 cells. Flow Cyt: SH-SY5Y cells.
General notes

This product was previously labelled as Metabotropic Glutamate Receptor 7

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

7G8D3
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [7G8D3] (ab233722)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Metabotropic Glutamate Receptor 7/MGLUR7 (green) using ab233722 at 1/200 dilution in ICC/IF.

Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [7G8D3] (ab233722)

Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [7G8D3] (ab233722) at 1/500 dilution + Recombinant human Metabotropic Glutamate Receptor 7/MGLUR7 (aa 454-590) protein

Predicted band size: 102 kDa

Expected MW is 41.7 kDa.
Western blot - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [7G8D3] (ab233722)

All lanes : Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [7G8D3] (ab233722) at 1/500 dilution

Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : Metabotropic Glutamate Receptor 7/MGLUR7 (aa 454-590)-hIgGFc transfected HEK-293 cell lysate

Predicted band size: 102 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [7G8D3] (ab233722)

SMMC-7721 (human hepatocarcinoma cell line) cells stained for Metabotropic Glutamate Receptor 7/MGLUR7 (green) using ab233722 at 1/200 dilution in ICC/IF.

Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [7G8D3] (ab233722)

Flow cytometric analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) cell line labeling Metabotropic Glutamate Receptor 7/MGLUR7 with ab233722 at 1/200 dilution (green) compared with a negative control (red).

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