Anti-IL-10 antibody [B-S10] - BSA and Azide free (ab269723)
![Anti-IL-10 antibody [B-S10] - BSA and Azide free (ab269723)](https://www.abcam.com/ps/products/269/ab269723/Images/ab269723-1-ab4722259611antiil10antibodybs10elisa.jpg "Anti-IL-10 antibody [B-S10] - BSA and Azide free (ab269723)")
Key features and details
- Mouse monoclonal [B-S10] to IL-10 - BSA and Azide free
- Suitable for: ELISA
- Reacts with: Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-IL-10 antibody [B-S10] - BSA and Azide free
See all IL-10 primary antibodies -
Description
Mouse monoclonal [B-S10] to IL-10 - BSA and Azide free -
Host species
Mouse -
Specificity
This antibody recognizes both natural and recombinant IL-10.
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Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragment
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Immunogen
Recombinant full length protein corresponding to Human IL-10.
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General notes
ab269723 is a carrier free version of ab47222. This format is designed for use in antibody labeling, including fluorochromes, metal isotypes, oligonucleotides, enzymes.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Purification notes
This antibody is sterile filtered through .22 µm and treated to remove endotoxins. It is carrier and preservative free. -
Clonality
Monoclonal -
Clone number
B-S10 -
Myeloma
P3x63-Ag8.653 -
Isotype
IgG1 -
Research areas
Images
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ELISA - Anti-IL-10 antibody (ab269723)We examined the effect of anti IL-10 to study the regulatory role of IL-10 and the interaction of IL-10 with its receptor. Measurement of neutralizing activity of anti IL-10 by TNFa-ELISA. IL-10 decreased the LPS-induced production of TNFa by Monocytes and recombinant IL-10 is neutralized by increasing concentrations of anti IL-10 antibody. The TNFa production showed a dependence on antibody concentration of anti IL-10.
ab47222 used at a 1/2000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab47222).
