Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1465Y] to Serine/threonine-protein kinase 4/MST-1
  • Suitable for: Flow Cyt, WB, IHC-P, ICC/IF, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y]
    See all Serine/threonine-protein kinase 4/MST-1 primary antibodies

  • Description

    Rabbit monoclonal [EP1465Y] to Serine/threonine-protein kinase 4/MST-1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Serine/threonine-protein kinase 4/MST-1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Jurkat, Ramos and HeLa cell lysate and mouse spleen, rat spleen and human urinary bladder tissues. ICC/IF: Raw264.7 cells. IHC-P:Human gastric carcinoma. IP: Jurkat cell lysate. Flow Cyt: HeLa cells.

  • General notes

    Previously labelled as Serine/threonine-protein kinase 4

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)
    Western blot - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)
    All lanes : Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : MST-1 knockout HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Ramos cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 56 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab51134 observed at 52 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab51134 Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] was shown to specifically react with MST-1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265442 (knockout cell lysate ab258215) was used. Wild-type and MST-1 knockout samples were subjected to SDS-PAGE. ab51134 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)
    Western blot - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)
    All lanes : Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134) at 1/50000 dilution

    Lane 1 : Jurkat (human acute T cell leukemia) whole cell lysate
    Lane 2 : Mouse spleen
    Lane 3 : Rat spleen

    Lysates/proteins at 1/20 dilution per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 56 kDa



    Diluting and blocking buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)
    Immunocytochemistry/ Immunofluorescence - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)

    ab51134 staining Serine/threonine-protein kinase 4/MST-1 in Raw264.7 (mouse abelson murine leukemia virus-induced tumor) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a concentration of 1/1000. ab7291 anti-Tubulin (mouse mAb) (1/1000) and ab150120 AlexaFluor®594 Goat anti-Mouse secondary (1/1000)  were used as counterstains for primary antibody ab51134 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)

    ab51134 staining Serine/threonine-protein kinase 4/MST-1 in human gastric carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Flow Cytometry - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)
    Flow Cytometry - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)

    Flow Cytometry analysis of HeLa cells labelling Serine/theronine-protein kinase 4/MST-1 with purified ab51134 at a dilution of 1/50 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Immunoprecipitation - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)
    Immunoprecipitation - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)

    ab51134 immunoprecipitating Serine/threonine-protein kinase 4/MST-1. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/30 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/1000.

    Lane 1: Jurkat (human acute T cell leukemia) whole cell lysate (10ug)
    Lane 2: Jurkat (human acute T cell leukemia) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab51134 in Jurkat (human acute T cell leukemia) whole cell lysate

  • Western blot - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)
    Western blot - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)
    Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134) at 1/10000 dilution + HeLa (human cervix adenocarcinoma) whole cell lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 56 kDa



    Diluting and blocking buffer: 5% NFDM/TBST

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