Anti-Serine/threonine-protein kinase 4/MST-1 antibody (ab264127)
Key features and details
- Rabbit polyclonal to Serine/threonine-protein kinase 4/MST-1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Serine/threonine-protein kinase 4/MST-1 antibody
See all Serine/threonine-protein kinase 4/MST-1 primary antibodies -
Description
Rabbit polyclonal to Serine/threonine-protein kinase 4/MST-1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
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Immunogen
Synthetic peptide within Human MST1 aa 375-425. The exact sequence is proprietary.
Database link: Q13043 -
Positive control
- WB: HeLa, Ramos and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Serine/threonine-protein kinase 4/MST-1 antibody (ab264127)All lanes : Anti-Serine/threonine-protein kinase 4/MST-1 antibody (ab264127) at 1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Predicted band size: 56 kDa
Exposure time: 30 seconds4-20% SDS-PAGE.
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Immunoprecipitation - Anti-Serine/threonine-protein kinase 4/MST-1 antibody (ab264127)Whole cell lysate (1 mg/IP reaction; 1/4 of reaction loaded/gel) from HeLa cells. ab264127 used at 3 μg/mg lysate for IP. MST1 and MST2 were simultaneously immunoprecipitated using MST1/2 antibodies.
Lane 1: ab264127.
Lane 2: MST1-2/STK3-4 antibody.
Lane 3: MST2/STK3 antibody.
Lane 4: Another MST1-2/STK3-4 antibody.
Lane 5: Control IgG.
Lane 6: Input.ab264127 is specific for Serine/threonine-protein kinase 4/MST-1.
