Recombinant Raf1 (mutated Y340E + Y341E) protein (Active) (ab62292)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Functional Studies
-
Product name
Recombinant Raf1 (mutated Y340E + Y341E) protein (Active)
See all Raf1 proteins and peptides -
Biological activity
The specific activity of RAF1 was determined to be ~6,000 nmol/min/mg in a coupled assay as per antibody assay protocol.
-
Purity
> 80 % SDS-PAGE.
Purity >80% as determined by SDS-PAGE and Coomassie blue staining -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Predicted molecular weight
63 kDa including tags -
Amino acids
306 to 648 -
Modifications
mutated Y340E + Y341E -
Tags
GST tag N-Terminus
-
Preparation and Storage
- c Raf
- C-raf
- C-Raf proto-oncogene, serine/threonine kinase
Images
-
Functional Studies - Recombinant Raf1 (mutated Y340E + Y341E) protein (Active) (ab62292)
ab62292 Kinase assay plot
-
Western blot - Recombinant Raf1 (mutated Y340E + Y341E) protein (Active) (ab62292)Recombinant Raf1 (mutated Y340E + Y341E) protein (Active) (ab62292) + RAF1
Predicted band size: 63 kDa
Observed band size: 63 kDa