Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1292] to BDNF - Low endotoxin, Azide free
  • Suitable for: IHC-P, Flow Cyt, ICC/IF, WB, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free
    See all BDNF primary antibodies

  • Description

    Rabbit monoclonal [EPR1292] to BDNF - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab182199)

  • Positive control

    • WB: Human, rat and mouse brain, hippocampus and cerebellum lysates; IHC-P: Human brain tissue, human bladder cancer tissue; Coronal sections from fetal (SNc) and post-natal (VTA) WT and Rgs6-/- mice.; ICC/IF: HeLa cells; Flow Cyt: HeLa cells; IHC-Fr: Mouse and Rat cerebrum tissue.

  • General notes

    ab206035 is the Low endotoxin, azide-free version of ab108319 This format is designed for in vitro and in vivo studies, including neutralization, blocking or activation/proliferation.

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)
    Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)

    Immunohistochemistry (Frozen sections) analysis of rat cerebral cortex tissue sections labeling BDNF with Purified ab108319 at 1/100 (2.8 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108319).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder cancer tissue sections labeling BDNF with Purified ab108319 at 1:500 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108319).

  • Flow Cytometry - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)
    Flow Cytometry - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BDNF with purified ab108319 at 1:30 dilution (10 µg/ml) (red). Cells were fixed with 80% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108319).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443) Image from Bifsha P et al. PLoS Genet 11(10) Fig 7C. doi: 10.1371/journal.pgen.1004863.

    Co-immunofluorescence staining against tyrosine hydroxylase (TH; green), nuclear Dapi (blue) and BDNF (red, ab108319) in coronal sections from fetal (SNc) and post-natal (VTA) WT and Rgs6−/− mice. Scale bar 20 µm.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108319).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)
    Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)

    Immunohistochemistry (Frozen sections) analysis of mouse cerebrum tissue sections labeling BDNF with Purified ab108319 at 1/100 (2.8 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108319).

  • Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)
    Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BDNF with Purified ab108319 at 1:500 (0.6 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108319).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)

    This IHC data was generated using the same anti-BDNF antibody clone, EPR1292, in a different buffer formulation (cat# ab108319).

    Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108319 at 1/100

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)
    Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443) This image is courtesy of an Abreview submitted by Kirk McManus.

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling BDNF with unpurified ab108319 at a dilution of 1/750. Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton-X100 in PBS. ab150081 (1/200) was used as the secondary antibody.

    The antibody produces a strong, golgi-associated labelling pattern in both PF and MeOH fixed samples.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108319).

  • Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)
    Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)

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