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Neuroscience Neurology process Growth and Development Neurotrophins

Anti-proBDNF antibody [BS375] (ab228505)

Anti-proBDNF antibody [BS375] (ab228505)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [BS375] to proBDNF
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-proBDNF antibody [BS375]
  • Description

    Mouse monoclonal [BS375] to proBDNF
  • Host species

    Mouse
  • Specificity

    The immunogen sequence for ab228505 corresponds to the propeptide and therefore this product is unable to detect mature BDNF.

  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human proBDNF aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P23560

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Flow Cyt: SH-SY5Y cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Lyophilized:Spin vial briefly before opening. Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives. After reconstitution divide into aliquots and store at -20ºC for a higher stability. Antibody contains no preservatives. Store at 4ºC with an appropriate antibacterial agent. Use sterile methods. Highest purity Glycerol (1:1) may be added for an additional stability when stored at refrigerated or freezing temperatures.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Constituents: PBS, 3% Trehalose

    Lyophilized without preservatives.
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    BS375
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Neuroscience
    • Neurology process
    • Growth and Development
    • Neurotrophins
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Huntington's disease
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cardiovascular
    • Atherosclerosis
    • Thrombosis
    • Other
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity

Images

  • Flow Cytometry - Anti-proBDNF antibody [BS375] (ab228505)
    Flow Cytometry - Anti-proBDNF antibody [BS375] (ab228505)

    Flow cytometric analysis of methanol-fixed (10 minutes on ice), 0.1% Tween-20-permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling BDNF with ab228505 at 2 µg per 106 cells (black) compared with a Rabbit IgG Control (white). Goat anti-Mouse PE at 1/100 dilution was used as the secondary antibody.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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