Human CDNF ELISA kit (ab215415)
Key features and details
- Sensitivity: 1 pg/ml
- Range: 15 pg/ml - 960 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CDNF ELISA kit
See all CDNF kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 15 677pg/ml 3.7% 2 15 272pg/ml 2.8% 3 15 167pg/ml 2.5% Inter-assay Sample n Mean SD CV% 1 2 19pg/ml 8.1% 2 2 155pg/ml 16% 3 2 439pg/ml 20.8% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1 pg/ml -
Range
15 pg/ml - 960 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Spike 92.9 0pg/ml - 960pg/ml -
Assay time
2h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The human CDNF ELISA kit (ab215415) provides a rapid and easy method for the quantitative determination of human CDNF in cell culture supernatant, serum and plasma. The kit includes ready-to-use reagents necessary to analyze up to 88 samples in 2 hours.
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Notes
Microplate wells are pre-coated with anti-human CDNF-specific monoclonal capture antibodies. Samples and standards are pipetted into microwells and human CDNF molecules present in the sample are bound by the capture antibodies. After incubation, unbound material is removed by washing the wells. Then, horseradish peroxidase (HRP) conjugated human CDNF-specific monoclonal detection antibodies bind to a different epitope of human CDNF molecules. After washing, the ready to use HRP substrate (TMB) is added to wells. The intensity of the color produced is directly proportional to the amount of human CDNF in the sample. Color development is then stopped by the addition of stop solution. Absorbance is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests anti-Human CDNF coated microplate (12 x 8 wells) 1 x 96 tests 10X Wash concentrate 1 x 50ml Human CDNF enzyme conjugate 1 x 12ml Human CDNF sample diluent 1 x 25ml Human CDNF standard A (0 pg/mL) 1 x 1ml Human CDNF standard B (15 pg/mL) 1 x 1ml Human CDNF standard C (30 pg/mL) 1 x 1ml Human CDNF standard D (60 pg/mL) 1 x 1ml Human CDNF standard E (120 pg/mL) 1 x 1ml Human CDNF standard F (240 pg/mL) 1 x 1ml Human CDNF standard G (480 pg/mL) 1 x 1ml Human CDNF standard H (960 pg/mL) 1 x 1ml Stop solution 1 x 12ml TMB Substrate solution 1 x 12ml -
Research areas
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Function
Trophic factor for dopamine neurons. Prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra. -
Tissue specificity
Widely expressed in neuronal and non-neuronal tissues. In the brain, highest levels in the optic nerve and corpus callosum. -
Sequence similarities
Belongs to the ARMET family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Arginine rich mutated in early stage tumors like 1
- ARMET L1
- ARMET like protein 1
see all -
Database links
- Entrez Gene: 441549 Human
- Omim: 611233 Human
- SwissProt: Q49AH0 Human
- Unigene: 559067 Human
Images
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Human CDNF ELISA kit ab215415. Linear regression (LLS) fit curve on a lin-lin scale
Human CDNF ELISA kit ab215415. Linear regression (LLS) fit curve on a lin-lin scale
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Human CDNF ELISA kit ab215415. Standard Curve, Cubic spline fit curve on a log-log scale.Human CDNF ELISA kit ab215415. Standard Curve, Cubic spline fit curve on a log-log scale.