Human CD13 ELISA Kit (ab239427)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 25.6 pg/ml
- Range: 219 pg/ml - 14000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CD13 ELISA Kit
See all CD13 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 8 4.5% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
25.6 pg/ml -
Range
219 pg/ml - 14000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 90 % - % Serum 110 % - % Hep Plasma 107 % - % EDTA Plasma 90 % - % Cit plasma 83 % - % -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CD13 ELISA Kit (ab239427) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD13 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD13 with 25.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CD13 Capture Antibody 1 x 600µl 10X Human CD13 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml Antibody Diluent 4BI 1 x 6ml Human CD13 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. -
Tissue specificity
Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients. -
Sequence similarities
Belongs to the peptidase M1 family. -
Domain
Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies). -
Post-translational
modificationsSulfated.
N- and O-glycosylated.
May undergo proteolysis and give rise to a soluble form. -
Cellular localization
Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected. - Information by UniProt
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Alternative names
- Alanyl (membrane) aminopeptidase
- Alanyl aminopeptidase
- Aminopeptidase M
see all -
Database links
- Entrez Gene: 290 Human
- Omim: 151530 Human
- SwissProt: P15144 Human
- Unigene: 1239 Human
Images
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Other - Human CD13 ELISA Kit (ab239427)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human CD13 standard curve in Sample Diluent NS + Enhancer.The CD13 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native CD13 in human serum and plasma samples.The concentrations of CD13 were measured in duplicates, interpolated from the CD13 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1: 160, plasma (citrate) 1: 80, plasma (heparin) 1: 80, and plasma (EDTA) 1: 80. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD13 concentration was determined to be 724.9 ng/mL in serum, 658.0 ng/mL in plasma (citrate), 651.2 ng/ml in plasma (heparin), and 618.4 ng/mL in plasma (EDTA). -
Interpolated concentrations of native CD13 in human HUVEC cell culture supernatant samples.The concentrations of CD13 were measured in duplicates, interpolated from the CD13 standard curves and corrected for sample dilution. Undiluted samples are as follows: HUVEC supernatant 1: 40. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean, dilution factor corrected CD13 concentration was determined to be 366.5 ng/ml in HUVEC supernatant. -
Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD13 concentration was determined to be 1245.6 ng/mL with a range of 1006.8 - 1765.3 ng/mL.
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Sandwich ELISA - Human CD13 ELISA Kit (ab239427)To learn more about the advantages of recombinant antibodies see here.