Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD64 antibody [10.1] (ab119843)

Key features and details

  • Mouse monoclonal [10.1] to CD64
  • Suitable for: Flow Cyt
  • Reacts with: Human, Non human primates
  • Isotype: IgG1

Overview

  • Product name

    Anti-CD64 antibody [10.1]
    See all CD64 primary antibodies

  • Description

    Mouse monoclonal [10.1] to CD64

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human, Non human primates

  • Immunogen

    Rheumatoid synovial fluid cells and fibronectin purified Human monocytes.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    ab119843 is purified from cell culture supernatant by protein-A affinity chromatography. Purity is > 95% by SDS-PAGE.

  • Clonality

    Monoclonal

  • Clone number

    10.1

  • Isotype

    IgG1

  • Research areas

Images

  • Flow Cytometry - Anti-CD64 antibody [10.1] (ab119843)
    Flow Cytometry - Anti-CD64 antibody [10.1] (ab119843)
    Surface staining of CD64 in human peripheral blood with anti-CD64 (10.1) purified , GAM-APC.
  • Flow Cytometry - Anti-CD64 antibody [10.1] (ab119843)
    Flow Cytometry - Anti-CD64 antibody [10.1] (ab119843)
    Overlay histogram showing THP1 cells stained with ab119843 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab119843, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed THP1 cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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