Anti-CD64 antibody [OTI3D3] (ab140779)
![Anti-CD64 antibody [OTI3D3] (ab140779)](https://www.abcam.com/ps/products/140/ab140779/Images/ab140779-156199-anti-cd64-antibody-oti3d3-immunohistochemistry.jpg "Anti-CD64 antibody [OTI3D3] (ab140779)")
Key features and details
- Mouse monoclonal [OTI3D3] to CD64
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-CD64 antibody [OTI3D3]
See all CD64 primary antibodies -
Description
Mouse monoclonal [OTI3D3] to CD64 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF African green monkeyIHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to amino acids 1-374 of Human CD64 produced in HEK293T cell (NP_000557).
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Positive control
- Recombinant Human CD64 protein (ab114416) can be used as a positive control in WB. HEK293T cells transfected with pCMV6-ENTRY CD64 cDNA, Human ovary adenocarcinoma tissue, Human lymphoma tissue, COS7 cells transiently transfected by pCMV6-ENTRY CD64
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General notes
The clone number has been updated from 3D3 to OTI3D3, both clone numbers name the same clone.
Dilute in PBS (pH 7.3) prior to use.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA, 48% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
OTI3D3 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [OTI3D3] (ab140779)Immunohistochemical analysis of paraffin embedded Human ovary adenocarcinoma tissue labelling CD64 with ab140779 at a 1/150 dilution.
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![Western blot - Anti-CD64 antibody [OTI3D3] (ab140779)](https://www.abcam.com/ps/products/140/ab140779/Images/ab140779-156200-anti-cd64-antibody-oti3d3-western-blot.jpg)
Western blot - Anti-CD64 antibody [OTI3D3] (ab140779)All lanes : Anti-CD64 antibody [OTI3D3] (ab140779) at 1/4000 dilution
Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cells transfected with pCMV6-ENTRY CD64 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 43 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC210076) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [OTI3D3] (ab140779)](https://www.abcam.com/ps/products/140/ab140779/Images/ab140779-156198-anti-cd64-antibody-oti3d3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [OTI3D3] (ab140779)Immunohistochemical analysis of paraffin embedded Human lymphoma tissue labelling CD64 with ab140779 at a 1/150 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-CD64 antibody [OTI3D3] (ab140779)](https://www.abcam.com/ps/products/140/ab140779/Images/ab140779-156197-anti-cd64-antibody-oti3d3-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD64 antibody [OTI3D3] (ab140779)Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD64 using ab140779 at a 1/100 dilution.
