Anti-CD64 antibody [EPR4623] (ab109449)
![Anti-CD64 antibody [EPR4623] (ab109449)](https://www.abcam.com/ps/products/109/ab109449/Images/ab109449-122317-anti-cd64-antibody-epr4623-flow-cytometry.jpg "Anti-CD64 antibody [EPR4623] (ab109449)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4623] to CD64
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CD64 antibody [EPR4623]
See all CD64 primary antibodies -
Description
Rabbit monoclonal [EPR4623] to CD64 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- U937, THP1, and HL60 cell lysates; U937 cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.00 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4623 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-CD64 antibody [EPR4623] (ab109449)
ab109449 at 1/100 dilution staining CD64 in U937 cells by Flow cytometry (shown in red). Rabbit IgG negative control (shown in green).
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![Western blot - Anti-CD64 antibody [EPR4623] (ab109449)](https://www.abcam.com/ps/products/109/ab109449/Images/ab109449-122318-anti-cd64-antibody-epr4623-western-blot.jpg)
Western blot - Anti-CD64 antibody [EPR4623] (ab109449)All lanes : Anti-CD64 antibody [EPR4623] (ab109449) at 1/1000 dilution
Lane 1 : U937 cell lysate
Lane 2 : THP1 cell lysate
Lane 3 : HL60 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDa
Observed band size: 64-78 kDa why is the actual band size different from the predicted?
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![OI-RD Scanning - Anti-CD64 antibody [EPR4623] (ab109449)](https://www.abcam.com/ps/products/109/ab109449/Images/ab109449-122316-anti-cd64-antibody-epr4623.jpg)
OI-RD Scanning - Anti-CD64 antibody [EPR4623] (ab109449) -
![Anti-CD64 antibody [EPR4623] (ab109449)](https://www.abcam.com/ps/products/109/ab109449/Images/ab109449-4-benefits-of-recombinant-antibodies.png)
Anti-CD64 antibody [EPR4623] (ab109449)
