Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD35 antibody [7G9] (ab254151)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [7G9] to CD35
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD35 antibody [7G9]
    See all CD35 primary antibodies

  • Description

    Mouse monoclonal [7G9] to CD35

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human CD35. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P17927

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • ICC/IF: Human peripheral blood mononuclear cells. Flow Cyt: Human peripheral blood mononuclear cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    CD35 is an alternative name for CR1.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CD35 antibody [7G9] (ab254151)
    Immunocytochemistry/ Immunofluorescence - Anti-CD35 antibody [7G9] (ab254151)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMC cells labelling CD35 / CR1 with ab254151 at 1/100 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous and cytoplasmic staining in human PBMC. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (Red). The nuclear counterstain was DAPI (Blue).

    Negative control 1: ab254151 at a 1/100 dilution followed by ab150080 at a 1/500 diliution.

    Negative control 2: ab179513 at a 1/500 dilution followed by ab150113 at a 1/1000 diliution.

  • Flow Cytometry - Anti-CD35 antibody [7G9] (ab254151)
    Flow Cytometry - Anti-CD35 antibody [7G9] (ab254151)

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling CD35 / CR1 with ab254151 at 1/1000 dilution (0.1µg) (Right panel) compared with a mouse monoclonal IgG isotype control (Left panel). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with mouse IgG (Left) or ab254151 (Right), then stained with anti-CD19 conjugated to Alexa Fluor® 647.

    Gated on viable cells. 

  • Anti-CD35 antibody [7G9] (ab254151)
    Anti-CD35 antibody [7G9] (ab254151)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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